CAJ | 학술논문

目的：观察低浓度84消毒液对小鼠生殖生理的影响。方法将72只KM小鼠随机分为3组，即：0.5％84消毒液组（ A组）、2.0％84消毒液组（ B组）和生理盐水组（ C组），每组24只，雌雄各半，各组每天1次灌胃给予相应药物，连续9周。阴道涂片镜检观察雌性小鼠的动情周期。9周后，观察雄性小鼠的精子畸形率；并用ELISA法测定小鼠血清中性激素的水平；并取睾丸（或卵巢）、心、肝、肺、肾进行组织学观察。结果与C组比较，A和B组小鼠体重差异无显著性（ P ＞0.05）；血清性激素水平差异无显著性（ P ＞0.05）；雌性小鼠动情周期差异无显著性（P ＞0.05）；组织学观察未见异常；与C组比较，A组小鼠精子畸形率差异无显著性（P ＞0.05），B组小鼠精子畸形率显著增高（ P ＝0.041＜0.05）。结论0.5％84消毒液对小鼠的生殖生理无明显影响，在实际使用中要严格控制消毒液浓度。
목적：관찰저농도84소독액대소서생식생리적영향。방법장72지KM소서수궤분위3조，즉：0.5％84소독액조（ A조）、2.0％84소독액조（ B조）화생리염수조（ C조），매조24지，자웅각반，각조매천1차관위급여상응약물，련속9주。음도도편경검관찰자성소서적동정주기。9주후，관찰웅성소서적정자기형솔；병용ELISA법측정소서혈청중성격소적수평；병취고환（혹란소）、심、간、폐、신진행조직학관찰。결과여C조비교，A화B조소서체중차이무현저성（ P ＞0.05）；혈청성격소수평차이무현저성（ P ＞0.05）；자성소서동정주기차이무현저성（P ＞0.05）；조직학관찰미견이상；여C조비교，A조소서정자기형솔차이무현저성（P ＞0.05），B조소서정자기형솔현저증고（ P ＝0.041＜0.05）。결론0.5％84소독액대소서적생식생리무명현영향，재실제사용중요엄격공제소독액농도。
Objective To study the effect of low-level 84 disinfection solution on reproductive physiology of mice . Methods 72 KM mice were randomly divided into three groups :0.5%84 disinfection solution group ( group A) , 2.0%84 disinfection solution group (group B) and saline solution group (group C).12 males and 12 females in each group. Corresponding medicine was given once a day by intragastric gavage for 9 weeks.The estrous cycle of female mice was observed by exfoliative cytology of vagina .After 9 weeks, The sperm abnormality test was performed , Serum samples were taken for sex hormone test by ELISA, and the histological change of testis (or ovary), heart, liver, lung and kidney were observed.Results Compared with group C , No significant difference was observed in the weight variation and serum sex hormone level in group A and B (P >0.05).The difference of the estrous cycle of female mice was not significant (P >0.05).No histopathological abnormality was observed .Compared with group C, There was no significant difference in the rate of sperm abnormality in group A ( P >0.05 ) , but the rate in group B was significantly increased ( P <0.05 ) . Conclusion 0.5% 84 disinfection solution has no significant effect on reproductive physiology of mice , The concentration of disinfection solution must be strictly controlled when used .