CAJ | 학술논문

目的：复制两种甲状腺功能亢进症动物模型，对比研究它们成型性及可持续性的异同，为研究治疗甲亢的药物提供良好的评价载体。方法优甲乐模型：大鼠灌胃给予优甲乐50μg/100 g鼠重共21 d，于末次给药后第1、7、14天分别于眼底取血和接取尿液，检测相关指标；小肠结肠炎耶尔森氏菌模型：大鼠尾静脉注射小肠结肠炎耶尔森氏菌，于第0、5、10、15、20天共注射5次，并于末次注射后第5、15、25天大鼠眼底取血和接取尿液，检测相关指标。结果优甲乐模型：造模后T3、T4、FT3、FT4显著升高，但造模型后1周即恢复正常，尿液代谢的PCA图中显示模型组整体代谢网络偏离空白组，2周后恢复正常；小肠结肠炎耶尔森氏菌模型：造模后第15天和25天时T3和T4升高，第25天时TSH显著升高，尿液代谢的PCA图中模型组偏离空白组，而第15天和25天时未回到空白组位置。结论优甲乐甲亢模型成型性好但恢复迅速，作为药效评价时适合预防给药；而小肠结肠炎耶尔森氏菌甲亢模型成型性好并且持续性较好，作为药效评价时可采取治疗给药方式。
목적：복제량충갑상선공능항진증동물모형，대비연구타문성형성급가지속성적이동，위연구치료갑항적약물제공량호적평개재체。방법우갑악모형：대서관위급여우갑악50μg/100 g서중공21 d，우말차급약후제1、7、14천분별우안저취혈화접취뇨액，검측상관지표；소장결장염야이삼씨균모형：대서미정맥주사소장결장염야이삼씨균，우제0、5、10、15、20천공주사5차，병우말차주사후제5、15、25천대서안저취혈화접취뇨액，검측상관지표。결과우갑악모형：조모후T3、T4、FT3、FT4현저승고，단조모형후1주즉회복정상，뇨액대사적PCA도중현시모형조정체대사망락편리공백조，2주후회복정상；소장결장염야이삼씨균모형：조모후제15천화25천시T3화T4승고，제25천시TSH현저승고，뇨액대사적PCA도중모형조편리공백조，이제15천화25천시미회도공백조위치。결론우갑악갑항모형성형성호단회복신속，작위약효평개시괄합예방급약；이소장결장염야이삼씨균갑항모형성형성호병차지속성교호，작위약효평개시가채취치료급약방식。
Objective To provide a good evaluation for research the hyperthyroidism drugs , two kinds of hyperthyroidism animal models were copied , and compared the similarities and differences of their formability and sustainability.Methods Euthyrox-induced model: The rat was taken Euthyrox (50μg· 100g-1 rat weight) for 21 d. And then the blood and urine were collected in 1 d, 7 d and 14 d after last time oral administration of Euthyrox;Yersiniaenterocolitica-induced model:the rats were injected Yersiniaenterocolitica (YE) in 0、5、10、15、20 (IOCV).And then the blood and urine were collected in 5d, 15d and 25d after last IOCV of YE.Results Euthyrox-induced model:T3, T4, FT3, FT4 were increased significantly , but returned normal 1 week later in the model group , PCA showed that model group deviated from the blank group , but recovered 2 weeks later; Yersiniaenterocolitica-induced model: T3 and T4 were increased significantly in 15 and 25 d, TSH significant raised in 25 d, the model group deviated from the blank group in PCA, and didn’t return to the blank group in 15d or 25d.Conclusion Euthyrox-induced modelhas good formability butrecovered quickly , which is suitable for prevention of drug delivery on efficacy evaluation; Yersiniaenterocolitica-induced model with good formability and sustainability could be used for treatment of drug delivery on efficacy evaluation .