CAJ | 학술논문

以褐藻酸钠为唯一碳源,从腐烂海带中筛选得到褐藻酸钠降解能力较强的菌株 H4,经生理生化和16S rDNA序列分析鉴定该菌株属于交替单胞菌属(Alteromonas)。对菌株H4的发酵条件优化研究表明, H4的较优培养基组成为(w/v)：褐藻酸钠0.6%、酵母粉0.5%、蛋白胨0.25%、NaCl 3%；较优培养条件为：培养温度28℃,初始pH7.5,接种量1.5%(v/v),培养时间48 h。Fe3+对交替单胞菌H4的产酶有明显的促进作用,这在其他褐藻酸裂解酶生产菌株中未见报道,而 Cu2+对褐藻酸裂解酶的抑制作用高达45.78%。在优化后的培养条件下,粗酶液酶活达到146.45 U/mL,较优化前提高了39.4%。
이갈조산납위유일탄원,종부란해대중사선득도갈조산납강해능력교강적균주 H4,경생리생화화16S rDNA서렬분석감정해균주속우교체단포균속(Alteromonas)。대균주H4적발효조건우화연구표명, H4적교우배양기조성위(w/v)：갈조산납0.6%、효모분0.5%、단백동0.25%、NaCl 3%；교우배양조건위：배양온도28℃,초시pH7.5,접충량1.5%(v/v),배양시간48 h。Fe3+대교체단포균H4적산매유명현적촉진작용,저재기타갈조산렬해매생산균주중미견보도,이 Cu2+대갈조산렬해매적억제작용고체45.78%。재우화후적배양조건하,조매액매활체도146.45 U/mL,교우화전제고료39.4%。
A screening work was carried out to obtain a bacterium with high alginate lyase activity by using alginate as sole source of carbon. The strain was identified asAlteromonas based on physiology and biochemistry analysis, 16S rDNA sequence analysis, and was namedAlteromonassp.H4. It was shown that the optimal culture medium composition of producing alginate lyase was as follows (w/v): alginate 0.6%, yeast extracts 0.5%, peptone 0.25% and NaCl 3%. The optimized culture condition was as follows: culture temperature 28℃, initial pH value 7.5, inoculum size 1.5%(v/v) and optimal harvest time 48 h. The activity of alginate lyase was activated by Fe3+, which hasn’t been reported in other alginate lyase production strains, and the inhibitory effect by Cu2+ was up to 45.78%. Under optimized conditions, the alginate lyase activity of the Alteromonas sp. H4 was146.45 U/ml, which was increased by 39.4% than that before optimization.