海洋科学
海洋科學
해양과학
MARINE SCIENCES
2014年
7期
7-13
,共7页
长臂虾科(Palaemonidae)%分子系统发育%16s rRNA基因
長臂蝦科(Palaemonidae)%分子繫統髮育%16s rRNA基因
장비하과(Palaemonidae)%분자계통발육%16s rRNA기인
palaemonidae%molecular phylogenetic%16s rRNA gene
长臂虾科(Caridea: Palaemonidae)是真虾类中最大的科级单元之一,为了探索长臂虾科内部的系统演化关系,作者采用从Genbank中下载的长臂虾科两亚科(Palaemoninae, Pontoniinae)14属30种长臂虾的线粒体16s rRNA基因序列片段,以鼓虾科(Alpheidae)细足鼓虾(Alpheus gracilipes)为外群分析长臂虾亚科和隐虾亚科之间的系统发育关系。在获得的466个序列位点中,变异位点263个,简约信息位点213个。DNA 序列分析显示,隐虾亚科的德曼隐虾属(Manipontonia)与长臂虾亚科的遗传距离(0.211)要小于其与所在亚科的遗传距离(0.245);此属在基于贝叶斯法(BI)和邻接法(NJ)构建的系统发育树中也与长臂虾亚科的种属聚合为一支,然后与隐虾亚科种属聚合的一支成为姊妹群。结合此属与长臂虾亚科尾瘦虾属(Urocaridella)形态相似的特征,建议对德曼隐虾属在长臂虾科中的分类地位进行重新评估。除隐虾亚科德曼隐虾属聚合到长臂虾亚科聚类簇外,两亚科所属的其他种属各自聚合为一支,在系统树中互为姊妹群,支持将长臂虾分为两亚科的分类系统。
長臂蝦科(Caridea: Palaemonidae)是真蝦類中最大的科級單元之一,為瞭探索長臂蝦科內部的繫統縯化關繫,作者採用從Genbank中下載的長臂蝦科兩亞科(Palaemoninae, Pontoniinae)14屬30種長臂蝦的線粒體16s rRNA基因序列片段,以鼓蝦科(Alpheidae)細足鼓蝦(Alpheus gracilipes)為外群分析長臂蝦亞科和隱蝦亞科之間的繫統髮育關繫。在穫得的466箇序列位點中,變異位點263箇,簡約信息位點213箇。DNA 序列分析顯示,隱蝦亞科的德曼隱蝦屬(Manipontonia)與長臂蝦亞科的遺傳距離(0.211)要小于其與所在亞科的遺傳距離(0.245);此屬在基于貝葉斯法(BI)和鄰接法(NJ)構建的繫統髮育樹中也與長臂蝦亞科的種屬聚閤為一支,然後與隱蝦亞科種屬聚閤的一支成為姊妹群。結閤此屬與長臂蝦亞科尾瘦蝦屬(Urocaridella)形態相似的特徵,建議對德曼隱蝦屬在長臂蝦科中的分類地位進行重新評估。除隱蝦亞科德曼隱蝦屬聚閤到長臂蝦亞科聚類簇外,兩亞科所屬的其他種屬各自聚閤為一支,在繫統樹中互為姊妹群,支持將長臂蝦分為兩亞科的分類繫統。
장비하과(Caridea: Palaemonidae)시진하류중최대적과급단원지일,위료탐색장비하과내부적계통연화관계,작자채용종Genbank중하재적장비하과량아과(Palaemoninae, Pontoniinae)14속30충장비하적선립체16s rRNA기인서렬편단,이고하과(Alpheidae)세족고하(Alpheus gracilipes)위외군분석장비하아과화은하아과지간적계통발육관계。재획득적466개서렬위점중,변이위점263개,간약신식위점213개。DNA 서렬분석현시,은하아과적덕만은하속(Manipontonia)여장비하아과적유전거리(0.211)요소우기여소재아과적유전거리(0.245);차속재기우패협사법(BI)화린접법(NJ)구건적계통발육수중야여장비하아과적충속취합위일지,연후여은하아과충속취합적일지성위자매군。결합차속여장비하아과미수하속(Urocaridella)형태상사적특정,건의대덕만은하속재장비하과중적분류지위진행중신평고。제은하아과덕만은하속취합도장비하아과취류족외,량아과소속적기타충속각자취합위일지,재계통수중호위자매군,지지장장비하분위량아과적분류계통。
The molecular phylogenetic relationship between the two subfamilies of Palaemonidae Rafinesque (Palaemoninae Rafinesqueand Pontoniinae Kingsley)were reconstructed based on partial sequences of the mitochondrial 16s rRNA gene retrieved from GenBank. The aligned sequences dataset contained 14 genera, 30 sequences of the mitochondrial 16s rRNA gene of palaemonid shrimps, and one species from Alpheidae Rafinesque, 9(Alpheus gracilipesStimpson), as negative control. Sequence analysis showed that there were 263 variable sites and 213 parsimony informative sites among the total 466 base sites in the aligned dataset. The average content of A+T was 66.3%, significantly higher than G+C, indicating that the base compositions were biased in favor of A+T. The DNA genetic distance analysis showed that the genusManipontoniaBruce had a smaller genetic distance to the subfamily Palaemoninae (0.211) than to its coordinal genera in subfamily Pontoniinae (0.245). It was consistent with the molecular phylogenetic tree constructed by Bayesian inference and Neighbor-joining methods. In the phylogenetic tree, the genusManipontoniaclustered in the assemblage of Palaemoninae rather than in the cluster of Pontoniinae. Furthermore, this genus is more similar to the Palaemoninae genusUrocaridellaBorradailein morphology. The morphology and molecular evidences suggested the systematic status of the genusManipontonia needs to be re-evaluated. GenusManipontonia andthe two clustered subfamilies are sister groups, supporting the two subfamilies scheme of Palaemonidae.