CAJ | 학술논문

对一株采自祁连山的疑似Agrocybe属真菌进行了分离鉴定及生物学特性研究。通过组织分离获得该菌纯培养物，提取其基因组DNA，以此为模板进行了ITS片段的扩增、测序，及基于ITS序列的菌株系统发育分析，同时对该菌进行了生物学特性研究。结果显示：从菌丝体中扩增获得了700 bp左右的ITS片段，系统发育树分析表明该真菌属于Agrocybe属，GenBank登录号为KF702393。该菌菌丝生长的最适温度为24℃，最适pH为7．0，较适碳氮源为葡萄糖和酵母粉，添加一定量的无机盐和维生素有助于菌丝的生长。
대일주채자기련산적의사Agrocybe속진균진행료분리감정급생물학특성연구。통과조직분리획득해균순배양물，제취기기인조DNA，이차위모판진행료ITS편단적확증、측서，급기우ITS서렬적균주계통발육분석，동시대해균진행료생물학특성연구。결과현시：종균사체중확증획득료700 bp좌우적ITS편단，계통발육수분석표명해진균속우Agrocybe속，GenBank등록호위KF702393。해균균사생장적최괄온도위24℃，최괄pH위7．0，교괄탄담원위포도당화효모분，첨가일정량적무궤염화유생소유조우균사적생장。
We isolated and identified fungus from wild environment to study its biological characteristics .The mycelium was iso-lated from the fruiting body .Genomic DNA was extracted from mycelium , then used as template for the ITS gene PCR am-plification, sequencing and phylogenetic analysis.We also studied the biological characteristics of mycelium .About 700 bp fragment was amplified and sequenced .Phylogenetic analysis indicated that the fungi belonged to the genus Agrocybe.Gen-Bank accession number was KF 702393.The optimum temperature and pH were 24℃and 7.0, respectively.The optimum carbon sources and nitrogen sources were glucose and dry yeast , respectively .The fungi can advance the growth of myceli-um by adding some inorganic salt and vitamins .