CAJ | 학술논문

室内条件下采用叶片药膜法分别测定氧化乐果和甲萘威对黄褐天幕毛虫（ Malacosoma neustria testa-cea）3龄幼虫的毒力；采用分光光度法测定2种药剂亚致死剂量对黄褐天幕毛虫的羧酸酯酶（CarE），谷胱甘肽S-转移酶（ GSTs）活性的影响。结果表明：氧化乐果对黄褐天幕毛虫的24、48 h的LC20分别为33．975、8．298 mg? L-1，甲萘威24、48 h的LC20分别为23．314、1．863 mg? L-1。经氧化乐果和甲萘威亚致死剂量处理后，幼虫体内的CarE在6～48 h内均被不同程度抑制，其中处理12 h活性降到最低，分别为对照的0．51、0．36倍；氧化乐果亚致死剂量对幼虫体内的GSTs表现为抑制-激活-抑制作用，在处理后24 h对该酶的激活作用最强，为同期对照的1．37倍。亚致死剂量甲萘威处理6～12 h内幼虫体内GSTs被激活，随后逐渐被抑制。
실내조건하채용협편약막법분별측정양화악과화갑내위대황갈천막모충（ Malacosoma neustria testa-cea）3령유충적독력；채용분광광도법측정2충약제아치사제량대황갈천막모충적최산지매（CarE），곡광감태S-전이매（ GSTs）활성적영향。결과표명：양화악과대황갈천막모충적24、48 h적LC20분별위33．975、8．298 mg? L-1，갑내위24、48 h적LC20분별위23．314、1．863 mg? L-1。경양화악과화갑내위아치사제량처리후，유충체내적CarE재6～48 h내균피불동정도억제，기중처리12 h활성강도최저，분별위대조적0．51、0．36배；양화악과아치사제량대유충체내적GSTs표현위억제-격활-억제작용，재처리후24 h대해매적격활작용최강，위동기대조적1．37배。아치사제량갑내위처리6～12 h내유충체내GSTs피격활，수후축점피억제。
We adopted leaf-dip method to evaluate omethoate and carbaryl against the third instar of Malacosoma neustir a testacea and used sepectrophotometry to assess the effect of sublethal concentration of the two pesticides on activities of carboxyles -terase and glutathione S-transferase.For 24 h and 48 h treatment, the LC20 of omethoate were 33.975 and 8.298 mg? L-1 , and the LC20 of carbaryl were 23.314 and 1.863 mg? L-1, respectively.The activities of CarE in lavae were inhibited at different level within 6-48 h treatment of sublethal concentration of omethoate and carbary .Compared to CK , the activities of CarE were decreased by 51%and 36%, respectively, after 12 h treatment, which were decreased to the lowest level .By the treatment of the sublethal concentration of omethoat , the activity of GSTs was inhibited firstly , then increased and in-hibited finally .After 24 h treatment with sublethal concentration of omethoate , the activity was increased by 1.37 times compared to that of CK , which was at highest level .Within 6-12 h of treatment with sublethal concentration of carbaryl , the activity of GSTs in larvae was increased firstly , and then decreased gradually .