东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2014年
7期
117-121,125
,共6页
韩志校%张泽坤%董研%杨敏生%Dietrich Ewald
韓誌校%張澤坤%董研%楊敏生%Dietrich Ewald
한지교%장택곤%동연%양민생%Dietrich Ewald
植物内生细菌%16S rDNA%组培苗%硬枝扦插%生根生长
植物內生細菌%16S rDNA%組培苗%硬枝扢插%生根生長
식물내생세균%16S rDNA%조배묘%경지천삽%생근생장
Endophytic bacteria%16S rDNA%Micropropagated shoots%Hardwood cutting%Growth
以从白杨杂种741杨内生菌中提取得到的菌株P22和S16为研究对象,通过16S rDNA分子检测证明它们分别属于类芽孢杆菌( Paenibacillus sp.)和寡养食单胞菌( Stenotrophomonas sp.)。研究了菌株P22和S16对促进近缘种植物生根及生长的能力。结果表明:菌株P22和S16对三倍体毛白杨组培苗、欧美杨107和旱柳硬枝插条生根和生长促进作用显著。证明这2株菌株为植物促生菌,可能具有固氮和诱导产生植物激素的作用,菌株P 22的促进生长能力更加显著。
以從白楊雜種741楊內生菌中提取得到的菌株P22和S16為研究對象,通過16S rDNA分子檢測證明它們分彆屬于類芽孢桿菌( Paenibacillus sp.)和寡養食單胞菌( Stenotrophomonas sp.)。研究瞭菌株P22和S16對促進近緣種植物生根及生長的能力。結果錶明:菌株P22和S16對三倍體毛白楊組培苗、歐美楊107和旱柳硬枝插條生根和生長促進作用顯著。證明這2株菌株為植物促生菌,可能具有固氮和誘導產生植物激素的作用,菌株P 22的促進生長能力更加顯著。
이종백양잡충741양내생균중제취득도적균주P22화S16위연구대상,통과16S rDNA분자검측증명타문분별속우류아포간균( Paenibacillus sp.)화과양식단포균( Stenotrophomonas sp.)。연구료균주P22화S16대촉진근연충식물생근급생장적능력。결과표명:균주P22화S16대삼배체모백양조배묘、구미양107화한류경지삽조생근화생장촉진작용현저。증명저2주균주위식물촉생균,가능구유고담화유도산생식물격소적작용,균주P 22적촉진생장능력경가현저。
We isolated Paenibacillus (P22) andS tenotrophomonas (S16) from Endophytic bacteria of the hybrid poplar 741. They respectively belonged to Paenibacillus sp.and Stenotrophomonas sp.by 16S rDNA molecular detection .Then, we studied the ability of strains P 22 and S16 for promoting rooting and growing on sibling species plants .P22 and S16 remark-ably promoted rooting and growing of tissue culture seedlings of Triploid Populus tomentosa and hardwood cuttings of Popu-lus×canadensis ‘Neva’ and Salix matsudana.Two strains were plant growth-promoting bacteria, and they might have the functions of nitrogen fixation and inducing generation of plant hormones .The capacity of promoting growth of P 22 was more remarkable .