CAJ | 학술논문

以芍药品种‘粉玉奴’（Paeonia lactiflora‘Fenyunu’）为母本，牡丹品种‘凤丹白’（Paeonia ostii‘Feng-danbai ’）为父本，进行远缘杂交，观察母本‘粉玉奴’开花习性和柱头可授期，对杂交亲本的花粉进行了生活力测定和电镜扫描观察，并对授粉后花粉萌发及花粉管伸长过程进行荧光显微观察。结果表明：母本‘粉玉奴’的最佳授粉时间为花开放后的4～5d；牡丹与芍药远缘杂交主要存在受精前障碍，‘凤丹白’花粉能够在芍药柱头上黏附并萌发，但是萌发时间滞后，花粉管的生长受到阻碍，并诱导柱头产生一系列的胼胝质反应，最终使花粉管难以穿过花柱进入子房完成受精。
이작약품충‘분옥노’（Paeonia lactiflora‘Fenyunu’）위모본，모단품충‘봉단백’（Paeonia ostii‘Feng-danbai ’）위부본，진행원연잡교，관찰모본‘분옥노’개화습성화주두가수기，대잡교친본적화분진행료생활력측정화전경소묘관찰，병대수분후화분맹발급화분관신장과정진행형광현미관찰。결과표명：모본‘분옥노’적최가수분시간위화개방후적4～5d；모단여작약원연잡교주요존재수정전장애，‘봉단백’화분능구재작약주두상점부병맹발，단시맹발시간체후，화분관적생장수도조애，병유도주두산생일계렬적변지질반응，최종사화분관난이천과화주진입자방완성수정。
The experiment was conducted to study the cross-compatibility between tree peony and herbaceous peony with Paeonia ostii ‘Fengdanbai ’ cross with Paeonia lactiflora‘Fenyunu ’ , and then observe flowering habit and stigma receptivity of fe-male parent .The pollen shape forms of both parents , as well as pollen tube behavior after pollination were observed .The best pollination period of P.lactiflora‘Fenyunu’ was 4-5 d after blooming.The pre-fertilization barriers caused the sterili-ty of the distant crosses between tree peony and herbaceous peony .Although the pollens of ‘Fengdanbai ’ were able to ger-minate on the stigma of any female parents that were used , the pollen tube elongating was hindered .The pollen induced the stigma producing series of callose reactions , which could not make the pollen tube into the ovary to complete fertilization through the style , ultimately.