CAJ | 학술논문

为了获得三色堇耐热性相关的基因HSP70基因，根据GenBank上登录的拟南芥、陆地棉等HSP70基因序列设计引物，用同源克隆法分离出大花三色堇HAR的HSP70基因片段，并进行测序与比对。结果表明：克隆得到基因片段，长度为882 bp，其序列与已公布的几种植物HSP70的编码区相似率平均为85．1％，上游引物含于基因片段中。初步证明此基因片段为三色堇HSP70基因片段。
위료획득삼색근내열성상관적기인HSP70기인，근거GenBank상등록적의남개、륙지면등HSP70기인서렬설계인물，용동원극륭법분리출대화삼색근HAR적HSP70기인편단，병진행측서여비대。결과표명：극륭득도기인편단，장도위882 bp，기서렬여이공포적궤충식물HSP70적편마구상사솔평균위85．1％，상유인물함우기인편단중。초보증명차기인편단위삼색근HSP70기인편단。
In order to obtain the heat resistance gene HSP 70 of Viola ×wittrockiana, the primers were designed based on the se-quence of the HSP 70 gene sequences of Arabidopsis thaliaan , Gossypium hirsutum and other plants in GenBank .The HSP70 gene fragment of pansy HAR were cloned by homology cloning and the fragment was sequenced .The length of gene fragment of HAR were 882 bp, the gene sequence shared 85.1%similar rate with the CDS of HSP 70, and the forward primer was in-cluded in the fragment.The results provided evidence that the gene fragment were the HSP 70 of pansy HAR.