CAJ | 학술논문

目的：探讨电针对5月龄APP/PS1转基因AD模型鼠学习记忆行为学及脑皮层Aβ水平的影响。方法：20只APP/PS1转基因阳性雄鼠随机分为模型组、电针组，每组各10只，APP/PS1转基因阴性雄鼠10只为对照组，电针组针刺“百会”、“涌泉”后接电针仪，疏密波，每次针刺持续15 min，隔日1次；对照组、模型组以相同方法用鼠袋束缚15 min，隔日1次，3组都持续5周，以Morris水迷宫检测小鼠学习记忆能力，用免疫组化方法检测脑皮层Aβ1-40、Aβ1-42的表达。结果：3组间的逃避潜伏期差异有统计学意义（P＜0．05），电针组与模型组之间的差异有统计学意义（ P＜0．05）。免疫组化电针组Aβ1-40、Aβ1-42的表达降低。结论：电针或许通过减少Aβ的生成来提高APP/PS1双转基因小鼠的学习记忆能力。
목적：탐토전침대5월령APP/PS1전기인AD모형서학습기억행위학급뇌피층Aβ수평적영향。방법：20지APP/PS1전기인양성웅서수궤분위모형조、전침조，매조각10지，APP/PS1전기인음성웅서10지위대조조，전침조침자“백회”、“용천”후접전침의，소밀파，매차침자지속15 min，격일1차；대조조、모형조이상동방법용서대속박15 min，격일1차，3조도지속5주，이Morris수미궁검측소서학습기억능력，용면역조화방법검측뇌피층Aβ1-40、Aβ1-42적표체。결과：3조간적도피잠복기차이유통계학의의（P＜0．05），전침조여모형조지간적차이유통계학의의（ P＜0．05）。면역조화전침조Aβ1-40、Aβ1-42적표체강저。결론：전침혹허통과감소Aβ적생성래제고APP/PS1쌍전기인소서적학습기억능력。
Objective:To explore the effect of electro -acupuncture on learning and memory behavior and the cerebral cortex Aβlevel in five months APP/PS1 transgenic AD mice .Methods:20 APP/PS1 transgenic posi-tive male mice were randomly divided into a model group and an electro -acupuncture group ,with 10 in each group .At the same time there were 10 APP/PS1 transgenic negative male mice in the normal control group .The electro-acupuncture group was acupunctured at“Baihui”and“Yongquan” ,then was adopted the intervention of electrical needle equipment which was dilatational wave , 15 min at a time ,every other day at a time ,treatment lasted five weeks .Finally the ability of learning and memory of all mice was tested by Morris water maze .Use immunohistochemical imaging to study the levels of cerebral Aβin mice.Results:Escape latency between three groups was statistically significant ( P<0 .05 );the difference between the model group and the electro -acu-puncture group was statistically significant (P<0.05).Expressions of Aβ1-40 and Aβ1-42 of the electro-acupuncture group were lower than those in the model group .Conclusion:Electro-acupuncture can probably improve learning and memory ability of APP/PS1 double transgenic mice through reducing the level of Aβ.