针灸临床杂志
針灸臨床雜誌
침구림상잡지
JOURNAL OF CLINICAL ACUPUNCTURE AND MOXIBUSTION
2014年
8期
60-63
,共4页
糖尿病%缺血性脑卒中%针康法%NF-κB-p65%TNF-α%大鼠
糖尿病%缺血性腦卒中%針康法%NF-κB-p65%TNF-α%大鼠
당뇨병%결혈성뇌졸중%침강법%NF-κB-p65%TNF-α%대서
Diabetes%Cerebral ischemic stroke%Acupuncture with rehabilitation therapy%NF -κB -p65%TNF-α%Rats
目的：通过建立糖尿病缺血性脑卒中大鼠模型，探讨针康法对大鼠外周血清NF-κB-p65、TNF-α表达的影响。方法：将192只大鼠随机分为空白组、假手术组、模型组、针刺组、康复组、针康组。采用高脂高糖饲料结合STZ注射及改良线栓法建立糖尿病缺血性脑卒中模型。观察不同的治疗方法对外周血清NF-κB-p65、TNF-α表达的影响。结果：与针康组比较，术后7天针刺组、康复组NF-κB-p65含量下降低于针康组（P＜0．01，P＜0．05）；术后14天、21天针刺组含量下降低于针康组（ P＜0．05）。与针康组比较，术后7天TNF-α针刺组、康复组含量明显下降（ P＜0．05）；术后14天针刺组含量下降明显低于针康组（P＜0．05）。结论：针康法能够降低糖尿病脑卒中大鼠血清炎性因子的表达，进而改善缺血性脑卒中后神经功能缺损及胰岛素抵抗。
目的：通過建立糖尿病缺血性腦卒中大鼠模型，探討針康法對大鼠外週血清NF-κB-p65、TNF-α錶達的影響。方法：將192隻大鼠隨機分為空白組、假手術組、模型組、針刺組、康複組、針康組。採用高脂高糖飼料結閤STZ註射及改良線栓法建立糖尿病缺血性腦卒中模型。觀察不同的治療方法對外週血清NF-κB-p65、TNF-α錶達的影響。結果：與針康組比較，術後7天針刺組、康複組NF-κB-p65含量下降低于針康組（P＜0．01，P＜0．05）；術後14天、21天針刺組含量下降低于針康組（ P＜0．05）。與針康組比較，術後7天TNF-α針刺組、康複組含量明顯下降（ P＜0．05）；術後14天針刺組含量下降明顯低于針康組（P＜0．05）。結論：針康法能夠降低糖尿病腦卒中大鼠血清炎性因子的錶達，進而改善缺血性腦卒中後神經功能缺損及胰島素牴抗。
목적：통과건립당뇨병결혈성뇌졸중대서모형，탐토침강법대대서외주혈청NF-κB-p65、TNF-α표체적영향。방법：장192지대서수궤분위공백조、가수술조、모형조、침자조、강복조、침강조。채용고지고당사료결합STZ주사급개량선전법건립당뇨병결혈성뇌졸중모형。관찰불동적치료방법대외주혈청NF-κB-p65、TNF-α표체적영향。결과：여침강조비교，술후7천침자조、강복조NF-κB-p65함량하강저우침강조（P＜0．01，P＜0．05）；술후14천、21천침자조함량하강저우침강조（ P＜0．05）。여침강조비교，술후7천TNF-α침자조、강복조함량명현하강（ P＜0．05）；술후14천침자조함량하강명현저우침강조（P＜0．05）。결론：침강법능구강저당뇨병뇌졸중대서혈청염성인자적표체，진이개선결혈성뇌졸중후신경공능결손급이도소저항。
Objective:To explore effects of cluster needling of scalp acupuncture combined with rehabilitation on the expression of peripheral serum NF -κB-p65 and TNF-αin diabetic rats with focal cerebral ischemia . Methods:The rats were randomly divided into six groups: blank group , sham operation , model group , acu-puncture group , rehabilitation group and acupuncture with rehabilitation group .The high fat diet combined with STZ injection and improved method for line filling were used to induce focal cerebral ischemia models in diabet -ic rats.Enzyme-Linked Immunosorbent Assay was used to check peripheral serum NF -κB-p65 and TNF-αin rats.Results:Compared with acupuncture with rehabilitation group ,after 7 days the expression of NF -κB-p65 in the acupuncture with rehabilitation group was significantly reduced compared with that in the acu -puncture group and rehabilitation group ( P<0.01,P<0.05); after 14 days and 21 days acupuncture group decreased less than acupuncture with rehabilitation group (P<0.05);after 7 days the expression of TNF-αin the acupuncture with rehabilitation group was significantly reduced compared with that in the acupuncture group and rehabilitation group ( P<0 .05 );after 14 days acupuncture group decreased less than acupuncture with re-habilitation group ( P<0 .05 ) .Conclusion:Diabetes combined with cerebral ischemia can cause the expressions of NF-κB-p65 and TNF-αto increase .Acupuncture combined with rehabilitation can reduce the expres-sions of NF-κB-p65 and TNF-α, thus improve nervous function defect and insulin resistance after diabetes with focal cerebral ischemia .
