CAJ | 학술논문

目的：通过与传统的比例法在新发涂阳病人中对结核分枝杆菌进行耐药检测比较，评价 HAIN（线性探针）用于检测原发性耐药结核的可行性。方法随机抽取我院2012年1月至2013年3月新发涂阳病人的痰标本，分别进行 HAIN 检测和酸性罗氏培养、比例法药敏试验，并对结果进行分析比较，共323例病人被纳入分析。结果以比例法为金标准，HAIN 方法对异烟肼（P ＝0．823）和利福平（P ＝1．000）的耐药检测结果与金标准的差异无统计学意义，HAIN 检测异烟肼耐药的灵敏性为80．4％、特异性为96．6％，检测利福平耐药的灵敏性为95．8％、99．6％。HAIN 对原发耐多药结核的检测（P ＝0．790）与金标准的差异也无统计学意义，灵敏性为78．9％、特异性为97．9％。结论HAIN 技术适用于临床原发耐药结核的快速筛查。
목적：통과여전통적비례법재신발도양병인중대결핵분지간균진행내약검측비교，평개 HAIN（선성탐침）용우검측원발성내약결핵적가행성。방법수궤추취아원2012년1월지2013년3월신발도양병인적담표본，분별진행 HAIN 검측화산성라씨배양、비례법약민시험，병대결과진행분석비교，공323례병인피납입분석。결과이비례법위금표준，HAIN 방법대이연정（P ＝0．823）화리복평（P ＝1．000）적내약검측결과여금표준적차이무통계학의의，HAIN 검측이연정내약적령민성위80．4％、특이성위96．6％，검측리복평내약적령민성위95．8％、99．6％。HAIN 대원발내다약결핵적검측（P ＝0．790）여금표준적차이야무통계학의의，령민성위78．9％、특이성위97．9％。결론HAIN 기술괄용우림상원발내약결핵적쾌속사사。
Objective To evaluate the rapid drug resistance detection of Mycobacterium tuberculosis by HAIN(line probe assay),we also did drug resistance tests using proportion method and analyzed their characteristics.Methods we selected new smear-positive TB cases from 2012 January to 2013 March in our hospital,,isolated strains from clinical specimens,used HAIN to find the strains which were drug resistant to INH、RIF,and the results were compared with proportion method ,323 cases were enrolled in the analysis.Results The proportion method was used as golden stand-ard,There was no significant difference on INH(P =0.823)and on RIF(P =1.000)between HAIN and gold standard. the sensitivity and specificity of HAIN in detecting INH resistant cases was 80.4% and 96.6% respectively,the sensi-tivity and specificity of HAIN in detecting RIF resistant cases was 95.8% and 99.6% respectively.There was no sig-nificant difference in detecting new MDR cases between HAIN and gold standard,the sensitivity and specificity of HAIN was 79.5% and 97.9%.Conclusion the HAIN technique was suitable as a first-line screening assay for new drug resistant TB.