CAJ | 학술논문

通过对盐胁迫不同时间点柽柳根和叶组织的7个转录组数据进行分析，鉴定了7条MYB基因（命名为ThMYB1～ThMYB7），这些基因编码蛋白氨基酸残基数为206～918 aa，编码蛋白的相对分子质量为22．9～75．9，理论等电点为5．06～9．71。进一步分析了7个ThMYB基因在盐碱胁迫（0．3 mol? L-1NaHCO3）不同时间点柽柳不同组织中的表达谱，结果表明：尽管这7个ThMYB蛋白长度差异较大、同源性较低，但除ThMYB7外的其他7个ThMYB基因的表达均受盐碱胁迫调控，能对盐碱胁迫作出应答。
통과대염협박불동시간점정류근화협조직적7개전록조수거진행분석，감정료7조MYB기인（명명위ThMYB1～ThMYB7），저사기인편마단백안기산잔기수위206～918 aa，편마단백적상대분자질량위22．9～75．9，이론등전점위5．06～9．71。진일보분석료7개ThMYB기인재염감협박（0．3 mol? L-1NaHCO3）불동시간점정류불동조직중적표체보，결과표명：진관저7개ThMYB단백장도차이교대、동원성교저，단제ThMYB7외적기타7개ThMYB기인적표체균수염감협박조공，능대염감협박작출응답。
Seven full length cDNAs of ThMYB ( named as ThMYB1-7) were cloned from seven transcriptome cDNA libraries of Tamarix hispida.These ThMYB genes encode proteins of 206-918 amino acid residues with the molecular mass of 22.9-75.9 kDa and theoretical isoelectric point of 5.06-9.71.The gene expression profiles in T.hispida responding to NaHCO 3 were further investigated by analyzing seven transcriptome data .All ThMYB ( excepted ThMYB7) expression were regulated by saline-alkali stress, although these ThMYB proteins shared different length and low homology .It suggested that the ThMYBs may involved in the saline-alkali stress tolerance of T.hispida.