CAJ | 학술논문

提出了一种电击转化枯草芽孢杆菌的方法。该方法通过调节细胞培养和电击转化过程中培养基和溶液成分来调节细胞渗透压，提高细胞在高电场强度电击后的存活率而达到提高转化效率的目的。优化前的转化率为5．0×105 cfu/μg，经优化后转化效率可达8×106 cfu/μg，比优化前提高了15倍。
제출료일충전격전화고초아포간균적방법。해방법통과조절세포배양화전격전화과정중배양기화용액성분래조절세포삼투압，제고세포재고전장강도전격후적존활솔이체도제고전화효솔적목적。우화전적전화솔위5．0×105 cfu/μg，경우화후전화효솔가체8×106 cfu/μg，비우화전제고료15배。
This article provides a method of electro-transformation in Bacillus Subtilis.This method is to regulate cell osmotic pressure and solution composition by regulating cell culture and shock in the process of transformation medium,for improving the survival rate after cells in high electric field in-tensity of electric shock and achieving the goal that improve the transformation efficiency.The trans-formation rate is 5.0 ×105cfu/μg before optimizing.After optimizing,the transformation efficiency is up to 8 ×106 cfu/μg,being 15 times higher than before optimization.It greatly promotes the conver-sion efficiency.