CAJ | 학술논문

京尼平是一种天然生物交联剂，且具有多种生理活性。本文以β-葡萄糖苷酶催化栀子苷水解制备京尼平为目标体系，证明了京尼平对β-D-葡萄糖苷酶具有竞争性抑制作用；考察了β-D-葡萄糖苷酶在不同有机相-水体系中的稳定性，发现其在正辛醇-水、正己醇-水体系中的稳定性均较好；测定了栀子苷在正辛醇-水和正己醇-水体系中的分配系数kD,gardenoside分别为0.17和0.76，而京尼平的分配系数kD,genipin为42.57和37.75；分别在水、正辛醇-水和正己醇-水体系中进行栀子苷水解制备京尼平的反应，当底物浓度为0.25μmol·ml-1时京尼平收率分别为89.17%、93.96%、90.16%；进一步提高底物浓度为2.0μmol·ml-1时，正辛醇-水体系中京尼平收率高达91.9%，较水相体系（79.7%）提高了12.2%。本文的研究结果证明了采用正辛醇-水两相反应体系可部分解除产物抑制，提高产物收率，并简化后续的产物分离。
경니평시일충천연생물교련제，차구유다충생리활성。본문이β-포도당감매최화치자감수해제비경니평위목표체계，증명료경니평대β-D-포도당감매구유경쟁성억제작용；고찰료β-D-포도당감매재불동유궤상-수체계중적은정성，발현기재정신순-수、정기순-수체계중적은정성균교호；측정료치자감재정신순-수화정기순-수체계중적분배계수kD,gardenoside분별위0.17화0.76，이경니평적분배계수kD,genipin위42.57화37.75；분별재수、정신순-수화정기순-수체계중진행치자감수해제비경니평적반응，당저물농도위0.25μmol·ml-1시경니평수솔분별위89.17%、93.96%、90.16%；진일보제고저물농도위2.0μmol·ml-1시，정신순-수체계중경니평수솔고체91.9%，교수상체계（79.7%）제고료12.2%。본문적연구결과증명료채용정신순-수량상반응체계가부분해제산물억제，제고산물수솔，병간화후속적산물분리。
Genipin is an important bio-crosslinking agent with multiple physiological activities. In this paper, the competitive inhibition for activity ofβ-glucosidase by genipin was demonstrated. The stabilities ofβ-glucosidase in different organic solvents were investigated. Stability ofβ-glucosidase was satisfactory in the octanol-water and hexanol-water medium. The partition coefficients for gardenoside in the octanol-water and hexanol-water system were 0.17 and 0.76, while those for genipin were 42.57 and 37.75 respectively. The process for preparation of genipin was carried out in water, octanol-water and hexanol-water respectively, while gardenoside concentration was 0.25μmol·ml-1. The obtained genipin yields were 89.17%, 93.96%and 90.16%respectively. Furthermore, the effect of gardenoside concentration on genipin yield was also investigated. While in the octanol-water system, conversion rate of genipin was as high as 91.9%at the gardenoside concentration of 2.0μmol·ml-1, an increase of 12.2%compared to that in water. Using the octanol-water medium was helpful for partially eliminating product inhibition, improving genipin yield and simplifying product recovery.