中华肝脏外科手术学电子杂志
中華肝髒外科手術學電子雜誌
중화간장외과수술학전자잡지
CHINESE JOURNAL OF HEPATIC SURGERY(ELECTRONIC EDITION)
2014年
4期
242-246
,共5页
姚志成%胡昆鹏%黄品助%陈新桂%黄河%王庆亮%杨培生%刘波
姚誌成%鬍昆鵬%黃品助%陳新桂%黃河%王慶亮%楊培生%劉波
요지성%호곤붕%황품조%진신계%황하%왕경량%양배생%류파
增生%肝%肝再生%三碘甲状腺原氨酸%小鼠
增生%肝%肝再生%三碘甲狀腺原氨痠%小鼠
증생%간%간재생%삼전갑상선원안산%소서
Hyperplasia%Liver%Liver regeneration%Triiodothyronine%Mice
目的:探讨外源性三碘甲状腺原氨酸(T3)对小鼠肝脏增生的影响。方法健康SPF级雌性C57BL/6小鼠45只,按随机数字表法随机分为A组、B组和对照组,每组15只。A、B组小鼠分别按每公斤体重10、5μg经腹腔内注射外源性T32 ml,对照组注射生理盐水2 ml。3组分别于处理后0、7、14、21、42 d时各处死3只小鼠。处死后测量小鼠全肝脏重量。采用免疫组织化学方法检测肝细胞增殖情况。实验数据间比较采用t检验或方差分析。结果与对照组相比,处理后7、14、21、42 d A组的肝脏重量明显增加(t=3.298,6.760,7.119,6.128;P<0.05),处理后14、21、42 d B组的肝脏重量明显增加(t=4.188,4.570,2.978;P<0.05)。处理后7、14、21、42 d A组增加的肝脏重量均比B组明显多(t=4.935,4.303,4.033,4.480;P<0.05)。其中处理后21 d,A、B组的肝脏重量增加至高峰(F=21.480,11.244;P<0.05)。与对照组相比,处理后0、7、14、21、42 d A组肝细胞数明显增加(t=28.383,23.842,40.194,31.059,15.841;P<0.05),B组的肝细胞数也明显增加(t=9.097,7.680,20.597,42.192,14.415;P<0.05);且A组增加的肝细胞数均比B组明显多(t=8.016,4.872,10.719,9.514,7.831;P<0.05)。其中处理后14 d,A组的肝细胞数增加至高峰(F=169.190,P<0.05);处理后21 d,B组的肝细胞数增加至高峰(F=90.460,P<0.05)。处理后A、B组肝细胞均发生广泛性增殖。结论外源性T3可有效促进小鼠肝脏增生,随着T3浓度的升高,增生更为明显。
目的:探討外源性三碘甲狀腺原氨痠(T3)對小鼠肝髒增生的影響。方法健康SPF級雌性C57BL/6小鼠45隻,按隨機數字錶法隨機分為A組、B組和對照組,每組15隻。A、B組小鼠分彆按每公斤體重10、5μg經腹腔內註射外源性T32 ml,對照組註射生理鹽水2 ml。3組分彆于處理後0、7、14、21、42 d時各處死3隻小鼠。處死後測量小鼠全肝髒重量。採用免疫組織化學方法檢測肝細胞增殖情況。實驗數據間比較採用t檢驗或方差分析。結果與對照組相比,處理後7、14、21、42 d A組的肝髒重量明顯增加(t=3.298,6.760,7.119,6.128;P<0.05),處理後14、21、42 d B組的肝髒重量明顯增加(t=4.188,4.570,2.978;P<0.05)。處理後7、14、21、42 d A組增加的肝髒重量均比B組明顯多(t=4.935,4.303,4.033,4.480;P<0.05)。其中處理後21 d,A、B組的肝髒重量增加至高峰(F=21.480,11.244;P<0.05)。與對照組相比,處理後0、7、14、21、42 d A組肝細胞數明顯增加(t=28.383,23.842,40.194,31.059,15.841;P<0.05),B組的肝細胞數也明顯增加(t=9.097,7.680,20.597,42.192,14.415;P<0.05);且A組增加的肝細胞數均比B組明顯多(t=8.016,4.872,10.719,9.514,7.831;P<0.05)。其中處理後14 d,A組的肝細胞數增加至高峰(F=169.190,P<0.05);處理後21 d,B組的肝細胞數增加至高峰(F=90.460,P<0.05)。處理後A、B組肝細胞均髮生廣汎性增殖。結論外源性T3可有效促進小鼠肝髒增生,隨著T3濃度的升高,增生更為明顯。
목적:탐토외원성삼전갑상선원안산(T3)대소서간장증생적영향。방법건강SPF급자성C57BL/6소서45지,안수궤수자표법수궤분위A조、B조화대조조,매조15지。A、B조소서분별안매공근체중10、5μg경복강내주사외원성T32 ml,대조조주사생리염수2 ml。3조분별우처리후0、7、14、21、42 d시각처사3지소서。처사후측량소서전간장중량。채용면역조직화학방법검측간세포증식정황。실험수거간비교채용t검험혹방차분석。결과여대조조상비,처리후7、14、21、42 d A조적간장중량명현증가(t=3.298,6.760,7.119,6.128;P<0.05),처리후14、21、42 d B조적간장중량명현증가(t=4.188,4.570,2.978;P<0.05)。처리후7、14、21、42 d A조증가적간장중량균비B조명현다(t=4.935,4.303,4.033,4.480;P<0.05)。기중처리후21 d,A、B조적간장중량증가지고봉(F=21.480,11.244;P<0.05)。여대조조상비,처리후0、7、14、21、42 d A조간세포수명현증가(t=28.383,23.842,40.194,31.059,15.841;P<0.05),B조적간세포수야명현증가(t=9.097,7.680,20.597,42.192,14.415;P<0.05);차A조증가적간세포수균비B조명현다(t=8.016,4.872,10.719,9.514,7.831;P<0.05)。기중처리후14 d,A조적간세포수증가지고봉(F=169.190,P<0.05);처리후21 d,B조적간세포수증가지고봉(F=90.460,P<0.05)。처리후A、B조간세포균발생엄범성증식。결론외원성T3가유효촉진소서간장증생,수착T3농도적승고,증생경위명현。
Objective To investigate the impact of exogenous of triiodothyronine (T3) on the liver hyperplasia of mouse. Methods Forty-ifve healthy speciifc pathogen free (SPF) C57BL/6 mice were divided into group A, B and control group using random number table method with 15 mice in each group. Mice in group A, B were respectively injected with 2 ml exogenous T3 solutions 10, 5μg/kg intraperitoneally. Mice in control group were injected with 2 ml normal saline. Three mice of each group were put to death respectively on day 0, 7, 14, 21, 42 after treatment. The total liver weight of the mice was measured after death. The proliferation of liver cells was detected by immunohistochemistry. The experimental data were compared using t test or analysis of variance. Results Compared with control group, the liver weight of mice in group A increased signiifcantly on day 7, 14, 21, 42 after treatment (t=3.298, 6.760, 7.119, 6.128;P<0.05) , and the liver weight of mice in group B increased signiifcantly on day 14, 21, 42 after treatment (t=4.188, 4.570, 2.978;P<0.05). The increased liver weight in group A was signiifcantly more than that in group B on day 7, 14, 21, 42 after treatment (t=4.935, 4.303, 4.033, 4.480;P<0.05). The liver weight in group A, B rose to the top on day 21 after treatment (F=21.480, 11.244;P<0.05). Compared with control group, the liver cell count in group A increased signiifcantly on day 0, 7, 14, 21, 42 after treatment (t=28.383, 23.842, 40.194, 31.059, 15.841;P<0.05), and the same with group B (t=9.097, 7.680, 20.597, 42.192, 14.415;P<0.05). The increased liver cell count in group A was signiifcantly more than that in group B (t=8.016, 4.872, 10.719, 9.514, 7.831;P<0.05). The liver cell count rose to the top in group A on day 14 after treatment (F=169.190, P<0.05) and rose to the top in group B on day 21 after treatment (F=90.460, P<0.05). Extensive proliferation of liver cells was observed both in group A and B after treatment. Conclusions Exogenous T3 can effectively promotes the liver hyperplasia of mouse, and the hyperplasia becomes more signiifcant as the T3 concentration rises.