CAJ | 학술논문

目的：探讨骨髓单核细胞（BMMNCs）移植对脊髓横断（SCT）大鼠脊髓组织中神经营养因子-3（NT-3）和突触素（SYN）表达的影响。方法：146只Winstar大鼠（SPF级，雌雄不限）随机分为3组：SCT组49只，建立SCT模型；实验组49只，建立SCT模型，给予BMMNCs移植治疗；假手术组48只，切除对应的椎板不损伤脊髓；各组大鼠分别于术后5、7、14和21d行后肢功能BBB评分，RT-PCR和免疫组织化学技术检测受损脊髓组织中 NT-3和SYN的表达。结果：术后5、7、14和21d各时间点各组大鼠后肢BBB评分比较，SCT组和实验组均低于假手术组（P＜0．05），且SCT组更低于实验组（P＜0．05）。术后5、7、14和21d各时间点NT-3阳性细胞数及NT-3 mRNA比较，SCT组和实验组均高于假手术组（P＜0．05），且SCT组更低于实验组（P＜0．05）。SYN阳性颗粒数及SYN mRNA比较，SCT组和实验组均低于假手术组（P＜0．05），且SCT 组更低于实验组（P＜0．05）。结论：BMMNCs能够上调局部损伤脊髓NT-3和SYN的表达。
목적：탐토골수단핵세포（BMMNCs）이식대척수횡단（SCT）대서척수조직중신경영양인자-3（NT-3）화돌촉소（SYN）표체적영향。방법：146지Winstar대서（SPF급，자웅불한）수궤분위3조：SCT조49지，건립SCT모형；실험조49지，건립SCT모형，급여BMMNCs이식치료；가수술조48지，절제대응적추판불손상척수；각조대서분별우술후5、7、14화21d행후지공능BBB평분，RT-PCR화면역조직화학기술검측수손척수조직중 NT-3화SYN적표체。결과：술후5、7、14화21d각시간점각조대서후지BBB평분비교，SCT조화실험조균저우가수술조（P＜0．05），차SCT조경저우실험조（P＜0．05）。술후5、7、14화21d각시간점NT-3양성세포수급NT-3 mRNA비교，SCT조화실험조균고우가수술조（P＜0．05），차SCT조경저우실험조（P＜0．05）。SYN양성과립수급SYN mRNA비교，SCT조화실험조균저우가수술조（P＜0．05），차SCT 조경저우실험조（P＜0．05）。결론：BMMNCs능구상조국부손상척수NT-3화SYN적표체。
Objective:To explore the effects of bone marrow mononuclear cells (BMMNCs) on the expression of neurotro-phin-3 (NT-3) and synaptophysin (SYN) in the injured spinal cord of rats with complete transection of the spinal cord (SCT) .Methods:The rat model of complete SCT was set up ,and 146 rats were divided randomly into Sham group (n=48) ,SCT model group (n=49) and experimental group (n=49) .Sham group ,removing the corresponding lamin and sutu-ring muscle and skin separately ;SCT model group ,establishing SCT models ;experimental group ,given BMMNCs transplan-tation in the SCT models .On the postoperative day (POD) 5 ,7 ,14 and 21 ,the rat hindlimb was scored by the scale of Bas-so ,Beattie and Bresnahan (BBB Grading) ,and the expression levels of NT-3 and SYN were detected by the immunohisto-chemistry and RT-PCR .Results:On the POD 5 ,7 ,14 and 21 ,BBB scores in experimental group and SCT model group were significantly lower than in Sham group (P<0 .05) ,and those in SCT model group were lower than in experimental group (P<0 .05) .RT-PCR and immunohistochemistry revealed the expression levels of NT-3 mRNA and number of NT-3 posi-tive cells in experimental group and SCT model group were significantly increased as compared with Sham group ( P<0 .05) ,and those in SCT group were higher than in experimental group (P<0 .05) .The positive particles of SYN and SYN mRNA in experimental group and SCT model group were significantly increased as compared with Sham group (P<0 .05) , and those in SCT model group were increased as compared with those in experimental group (P< 0 .05) .Conclusions :BMMNCs can promote the expression of NT-3 and SYN in injured spinal cord tissues ,which may be one of the mechanisms by which BMMNCs repair spinal cord injury .