CAJ | 학술논문

目的：探讨奥氮平所致糖脂代谢紊乱对大鼠认知功能的影响。方法选择健康清洁级SD雄性大鼠40只，用随机数字表法分为正常对照组和奥氮平组，每组10只。奥氮平组大鼠用奥氮平1.1 mg/kg灌胃，1次/d，共4周，复制肥胖模型；正常对照组大鼠用等体积0.9%氯化钠注射液灌胃，1次/d，共4周。灌胃期间每周测量大鼠体重。第4周灌胃结束后，检测大鼠空腹血糖、总胆固醇（ TC）、三酰甘油（ TG）、高密度脂蛋白胆固醇（ HDL-C）和低密度脂蛋白胆固醇（ LDL-C）水平；行Y迷宫实验，记录并比较1组大鼠学习和记忆能力测试中达到＂学会标准＂所需电击次数；行Morris水迷宫实验，记录并比较1组大鼠的逃避潜伏期。迷宫实验结束后观察1组大鼠海马CA1区组织形态学变化。结果奥氮平组大鼠灌胃第1、3、4周体重均高于同期正常对照组（均 P﹤0.05），第4周时体重超过正常对照组平均体重的10%（P﹤0.05）。灌胃4周后，奥氮平组大鼠空腹血糖和血清TG、TC、LDL-C水平明显高于对照组，HDL-C明显低于正常对照组，差异均有统计学意义（均P﹤0.05）。Y迷宫实验显示奥氮平组大鼠所需电击次数明显多于正常对照组[（81.65±0.11）次比（50.15±0.01）次，P﹤0.05]；Morris水迷宫实验显示奥氮平组大鼠逃避潜伏期明显长于正常对照组[（61.10±0.10）s比（13.54±0.11）s，P﹤0.05]。灌胃4周后奥氮平组大脑海马CA1区神经细胞出现排列不齐、缺失和水肿等变化。结论奥氮平可致大鼠血糖和血脂水平升高并影响认知功能。
목적：탐토오담평소치당지대사문란대대서인지공능적영향。방법선택건강청길급SD웅성대서40지，용수궤수자표법분위정상대조조화오담평조，매조10지。오담평조대서용오담평1.1 mg/kg관위，1차/d，공4주，복제비반모형；정상대조조대서용등체적0.9%록화납주사액관위，1차/d，공4주。관위기간매주측량대서체중。제4주관위결속후，검측대서공복혈당、총담고순（ TC）、삼선감유（ TG）、고밀도지단백담고순（ HDL-C）화저밀도지단백담고순（ LDL-C）수평；행Y미궁실험，기록병비교1조대서학습화기억능력측시중체도＂학회표준＂소수전격차수；행Morris수미궁실험，기록병비교1조대서적도피잠복기。미궁실험결속후관찰1조대서해마CA1구조직형태학변화。결과오담평조대서관위제1、3、4주체중균고우동기정상대조조（균 P﹤0.05），제4주시체중초과정상대조조평균체중적10%（P﹤0.05）。관위4주후，오담평조대서공복혈당화혈청TG、TC、LDL-C수평명현고우대조조，HDL-C명현저우정상대조조，차이균유통계학의의（균P﹤0.05）。Y미궁실험현시오담평조대서소수전격차수명현다우정상대조조[（81.65±0.11）차비（50.15±0.01）차，P﹤0.05]；Morris수미궁실험현시오담평조대서도피잠복기명현장우정상대조조[（61.10±0.10）s비（13.54±0.11）s，P﹤0.05]。관위4주후오담평조대뇌해마CA1구신경세포출현배렬불제、결실화수종등변화。결론오담평가치대서혈당화혈지수평승고병영향인지공능。
Objective To investigate the effects of glucose and lipid metabolism disorder induced by olanzapine on cognitive function in rats. Methods Forty healthy male SD rats of clean degree were divided into the olanzapine group and the control group using random-digital table,each group comprised 10 rats. The rats in the olanzapine group received intragastric administration of olanzapine 1. 1 mg/kg once daily for 4 weeks for duplicating adiposity model. The rats in the control group received intragastric administration of same volume of 0. 9% sodium chloride solution once daily for 4 weeks. The rats' body weight in the 1 groups were measured every week during the intragastric administration. The levels of serum fasting blood-glucose,total cholesterol( TC),triacylglycerol( TG),high density lipoprotein cholesterol( HDL-C),and low density lipoprotein cholesterol( LDL-C ) in rats in the 1 groups were measured after the intragastric administration. The Y maze test was carried on and the number of shocks which was the criterion of learning ability were recorded and compared between the 1 groups. The Morris water maze was carried on and the escape latency was recorded and compared between the 1 groups. The morphological changes in hippocampal CA1 area in the 1 groups were observed after the maze experiments. Results The body weight on the second,third,and fourth weeks of intragastric administration in the olanzapine group was higher than those in the control group(all P﹤0. 05). The body weight on the fourth week of intragastric administration in the olanzapine group was 10% more than that in the control group(P ﹤0. 05). The levels of serum fasting glucose,TC,TG,and LDL-C in the olanzapine group were obviously higher than those in the control group, the level of serum HDL-C was significantly lower than that in the control group( all P﹤0. 05 )at the end of the four weeks of intragastric administration. The result of Y maze test showed the number of shocks in the olanzapine group was obviously more than that in the control group[(81. 65 ± 0. 11)times vs. (50. 15 ± 0. 01)times,P﹤0. 05]. The result of Morris water maze test showed the escape latency in the olanzapine group was longer than that in the control group[(61. 10 ± 0. 10)s vs.(13. 54 ± 0. 11)s,P﹤0. 05]. The morphological experiment showed the malalignment,deletion,and edema appeared in the hippocampal CA1 area neurons in the olanzapine group at the end of four weeks of intragastric administration. Conclusion Olanzapine can increase the levels of rats' blood-glucose and blood lipid,and affect the cognitive function.