水科学与工程技术
水科學與工程技術
수과학여공정기술
WATER SCIENCES AND ENGINEERING TECHNOLOGY
2014年
1期
7-9,10
,共4页
有机提取物%彗星实验%DNA损伤%遗传毒性
有機提取物%彗星實驗%DNA損傷%遺傳毒性
유궤제취물%혜성실험%DNA손상%유전독성
organic extracts%comet assay%DNA damages%genotoxicity
为研究水库水经国内常规给水处理工艺的各阶段出水中有机提取物对细胞DNA损伤的情况,于8月和1月取水库水作为原水,设定采集4个水样:原水(A)、经混凝沉淀后的沉后水(B)、滤后水(C)和经氯消毒的出水(D)。提取水样中的有机物配成不同浓度溶液,喂食小白鼠,取小白鼠脾脏细胞做彗星实验。结果无论在枯水期还是丰水期,水处理工艺各阶段出水对DNA都有不同程度的损伤,损伤程度顺序:A>B>D>C;丰水期水样对DNA损伤程度高于枯水期水样对DNA的损伤。结果表明,水库水中的有机物污染物具有一定的遗传毒性,常规给水处理工艺对遗传毒性有削减作用,但不能消除。加氯消毒增加了水库水的遗传毒性。
為研究水庫水經國內常規給水處理工藝的各階段齣水中有機提取物對細胞DNA損傷的情況,于8月和1月取水庫水作為原水,設定採集4箇水樣:原水(A)、經混凝沉澱後的沉後水(B)、濾後水(C)和經氯消毒的齣水(D)。提取水樣中的有機物配成不同濃度溶液,餵食小白鼠,取小白鼠脾髒細胞做彗星實驗。結果無論在枯水期還是豐水期,水處理工藝各階段齣水對DNA都有不同程度的損傷,損傷程度順序:A>B>D>C;豐水期水樣對DNA損傷程度高于枯水期水樣對DNA的損傷。結果錶明,水庫水中的有機物汙染物具有一定的遺傳毒性,常規給水處理工藝對遺傳毒性有削減作用,但不能消除。加氯消毒增加瞭水庫水的遺傳毒性。
위연구수고수경국내상규급수처리공예적각계단출수중유궤제취물대세포DNA손상적정황,우8월화1월취수고수작위원수,설정채집4개수양:원수(A)、경혼응침정후적침후수(B)、려후수(C)화경록소독적출수(D)。제취수양중적유궤물배성불동농도용액,위식소백서,취소백서비장세포주혜성실험。결과무론재고수기환시봉수기,수처리공예각계단출수대DNA도유불동정도적손상,손상정도순서:A>B>D>C;봉수기수양대DNA손상정도고우고수기수양대DNA적손상。결과표명,수고수중적유궤물오염물구유일정적유전독성,상규급수처리공예대유전독성유삭감작용,단불능소제。가록소독증가료수고수적유전독성。
Objective to investigate the effect of the reservoir water in different periods through the stages of conventional water treatment process on cell DNA damage. The raw water was collected in August and January in reservoir water. The water treatment process is accordant to international common technique for water treatment. Four water samples were col-lected:source water(A), settled water after the addition of coagulant(B), filtered water after sand filter(C), finished water disinfected with Cl2,(D). Organic exttacts from water samples were configured into high concentration, medium concentra-tion and low concentration to feed female mice. Continued the experiment for five days, then killed the mice, and got lym-phocytes of the mice to do the comet assay. Both in the dry season and wet season , water samples in different stages of the water treatment process have different degree of damage on DNA, and the order DNA damage degree: A>B>D>C; Alto-gether, the DNA damage degree of the water in wet season is higher than that of the water in dry season. Reservoir water have certain genetic toxicity because of organic pollutants in the water, the conventional water treatment process on genetic toxicity has cut effect, but does not eliminate. Chlorine disinfection increased the genetic toxicity of water. The genetic tox-icity of reservoir in wet season is higher than that in dry season.