CAJ | 학술논문

目的：探讨氟伐他汀对大鼠角膜碱烧伤后Smad-3表达的影响。方法24只SD大鼠随机分为3组：生理盐水组、氟伐他汀组和地塞米松组，每组8只，用1mol/L的NaOH烧伤大鼠右眼，生理盐水组、氟伐他汀组和地塞米松组分别以生理盐水、氟伐他汀和1g/L地塞米松点眼。烧伤后1～7 d用裂隙灯观察角膜新生血管的生长情况，HE染色观察角膜组织病理学改变，免疫组化观察Smad-3的表达情况。结果烧伤后第7天，氟伐他汀组的角膜新生血管生长密度显著低于生理盐水组，差异有统计学意义， P ＜0．05。组织病理学检查结果提示，氟伐他汀组与生理盐水组相比，新生血管的形成明显减少，管腔小且稀疏，周围炎症细胞浸润明显减少，角膜组织结构明显整齐。免疫组化结果显示氟伐他汀组的Smad-3的表达情况显著降低。结论氟伐他汀能通过下调Smad-3的表达，来减轻大鼠角膜碱烧伤后新生血管的生成。
목적：탐토불벌타정대대서각막감소상후Smad-3표체적영향。방법24지SD대서수궤분위3조：생리염수조、불벌타정조화지새미송조，매조8지，용1mol/L적NaOH소상대서우안，생리염수조、불벌타정조화지새미송조분별이생리염수、불벌타정화1g/L지새미송점안。소상후1～7 d용렬극등관찰각막신생혈관적생장정황，HE염색관찰각막조직병이학개변，면역조화관찰Smad-3적표체정황。결과소상후제7천，불벌타정조적각막신생혈관생장밀도현저저우생리염수조，차이유통계학의의， P ＜0．05。조직병이학검사결과제시，불벌타정조여생리염수조상비，신생혈관적형성명현감소，관강소차희소，주위염증세포침윤명현감소，각막조직결구명현정제。면역조화결과현시불벌타정조적Smad-3적표체정황현저강저。결론불벌타정능통과하조Smad-3적표체，래감경대서각막감소상후신생혈관적생성。
Objective To investigate the effects of fluvastatin on expression of Smad-3 in alkali-burned cornea in rats.Methods Alkali corneal burns were induced to right eye in 24 SD rats using 1 mol/L NaOH.Animals were then randomly received one of 3 treatments ( n =8 for each group): normal saline, fluvastatin, or dexamethasone.In 1~7 days, corneal neovascularization was examined with slit-lamp, and pathological changes were evaluated with HE staining . The expression of Smad-3 was detected with immunohistochemical staining .Results At 7 days after corneal burns , corne-al neovascularization was significantly less severe in rats treated with fluvastatin than in rats treated with normal saline ( P<0.05).Histopathological examines showed less neovascularization and inflammatory cells infiltration , and more orderly corneal structure .Immunohistochemical staining showed that the expression of Smad-3 was significantly lower in fluvastatin treated rats .Conclusion Fluvastatin can reduce the severity of corneal neovascularization , and the mechanism may be re-lated to the down-regulated expression of Smad-3.