CAJ | 학술논문

目的：通过观察面神经损伤后神经元型一氧化氮合酶（neuronal NOS, nNOS）在面神经核团中的表达，探讨NO在面神经损伤后的作用。方法：健康成年SD大白鼠20只，其中5只作为正常对照。其余15只为面神经损伤组，在双侧面神经下颊支制作挤压伤。术后1、2、3、4、5周各取3只损伤组大鼠作面神经核团nNOS表达的免疫组化观察。结果：正常情况下面神经元不表达nNOS。面神经损伤后，出现运动神经元变性，其神经元细胞nNOS免疫染色为阳性，并持续至损伤后第5周。结论：面神经损伤后其运动神经元的核团中会出现nNOS的表达。
목적：통과관찰면신경손상후신경원형일양화담합매（neuronal NOS, nNOS）재면신경핵단중적표체，탐토NO재면신경손상후적작용。방법：건강성년SD대백서20지，기중5지작위정상대조。기여15지위면신경손상조，재쌍측면신경하협지제작제압상。술후1、2、3、4、5주각취3지손상조대서작면신경핵단nNOS표체적면역조화관찰。결과：정상정황하면신경원불표체nNOS。면신경손상후，출현운동신경원변성，기신경원세포nNOS면역염색위양성，병지속지손상후제5주。결론：면신경손상후기운동신경원적핵단중회출현nNOS적표체。
Objective: To observe the variation of neuronal nitric oxide synthase (nNOS) expression in injured facial nerve tissue. Method: 20 adult male SD rats were used in this study, among them , 5 rats were normal controls. In experiment group, a compression injury was applied at one branch of bilateral facial nerve. Every 3 rats in experiment group were sacrified for nNOS immunostaining in a period of 1, 2, 3, 4 and 5 weeks respectively. Results:In normal facial nerve tissue, nNOS expression was not detected. In the injured facial nerve neurons, nNOS immunostaining expression was positive, and lasting up till the fifth weeks. Conclusion: After the nerve injury, the expressing of nNOS was observed in the facial nucleus, which may be related to the death of neuron.