口腔颌面外科杂志
口腔頜麵外科雜誌
구강합면외과잡지
CHINESE JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
2014年
4期
266-271
,共6页
于佳%郝永明%陆家瑜%邹德荣
于佳%郝永明%陸傢瑜%鄒德榮
우가%학영명%륙가유%추덕영
富血小板纤维蛋白(PRF)%骨髓间充质干细胞(BMSCs)%颊侧牙槽骨缺损%组织工程%犬
富血小闆纖維蛋白(PRF)%骨髓間充質榦細胞(BMSCs)%頰側牙槽骨缺損%組織工程%犬
부혈소판섬유단백(PRF)%골수간충질간세포(BMSCs)%협측아조골결손%조직공정%견
platelet-rich fibrin (PRF)%bone marrow mesenchymal stem cells (BMSCs)%buccal alveolar bone defects%tissue engineering%dog
目的:评价富血小板纤维蛋白(PRF)构建的组织工程骨,在颊侧骨壁缺损修复中的成骨作用。方法:体外将PRF作用于比格犬的骨髓间充质干细胞(BMSCs),第21天测定钙结节量;4、7、11 d时采用实时定量PCR法检测成骨相关基因。9只成年比格犬拔除上颌左右两侧的侧切牙,构建颊侧骨壁缺损的拔牙位点,并随机分为血凝块组、PRF组和组织工程骨组,在手术后即刻、6周及12周时评价各组的颊侧骨高度和拔牙窝的骨密度。结果:PRF在体外实验中能明显促进BMSCs的增殖,提高成骨分化以及钙化能力(P<0.05)。动物实验中,PRF表现出了促进早期伤口愈合的能力。在颊侧骨壁缺损的修复中,组织工程组在6周时骨高度和骨密度分别为(2.63±0.57) mm、(765.19±59.70) HU,较血凝块组[(5.65±2.91) mm、(599.08±53.88) HU]和PRF组[(4.14±1.16) mm、(644.76±65.39) HU]显著增高(P<0.05)。结论:PRF复合BMSCs构建的组织工程骨可以在早期有效地修复拔牙窝骨壁缺损。
目的:評價富血小闆纖維蛋白(PRF)構建的組織工程骨,在頰側骨壁缺損脩複中的成骨作用。方法:體外將PRF作用于比格犬的骨髓間充質榦細胞(BMSCs),第21天測定鈣結節量;4、7、11 d時採用實時定量PCR法檢測成骨相關基因。9隻成年比格犬拔除上頜左右兩側的側切牙,構建頰側骨壁缺損的拔牙位點,併隨機分為血凝塊組、PRF組和組織工程骨組,在手術後即刻、6週及12週時評價各組的頰側骨高度和拔牙窩的骨密度。結果:PRF在體外實驗中能明顯促進BMSCs的增殖,提高成骨分化以及鈣化能力(P<0.05)。動物實驗中,PRF錶現齣瞭促進早期傷口愈閤的能力。在頰側骨壁缺損的脩複中,組織工程組在6週時骨高度和骨密度分彆為(2.63±0.57) mm、(765.19±59.70) HU,較血凝塊組[(5.65±2.91) mm、(599.08±53.88) HU]和PRF組[(4.14±1.16) mm、(644.76±65.39) HU]顯著增高(P<0.05)。結論:PRF複閤BMSCs構建的組織工程骨可以在早期有效地脩複拔牙窩骨壁缺損。
목적:평개부혈소판섬유단백(PRF)구건적조직공정골,재협측골벽결손수복중적성골작용。방법:체외장PRF작용우비격견적골수간충질간세포(BMSCs),제21천측정개결절량;4、7、11 d시채용실시정량PCR법검측성골상관기인。9지성년비격견발제상합좌우량측적측절아,구건협측골벽결손적발아위점,병수궤분위혈응괴조、PRF조화조직공정골조,재수술후즉각、6주급12주시평개각조적협측골고도화발아와적골밀도。결과:PRF재체외실험중능명현촉진BMSCs적증식,제고성골분화이급개화능력(P<0.05)。동물실험중,PRF표현출료촉진조기상구유합적능력。재협측골벽결손적수복중,조직공정조재6주시골고도화골밀도분별위(2.63±0.57) mm、(765.19±59.70) HU,교혈응괴조[(5.65±2.91) mm、(599.08±53.88) HU]화PRF조[(4.14±1.16) mm、(644.76±65.39) HU]현저증고(P<0.05)。결론:PRF복합BMSCs구건적조직공정골가이재조기유효지수복발아와골벽결손。
Objective: To evaluate platelet-rich fibrin (PRF) scaffolds and BMSCs in repairing mandibular buccal bone defects at canine′s tooth sockets. Methods:Fabrication of PRF were combined with beagle′s BMSCs scaffolds in vitro, and in day 21, calcium nodules were detected. Real time PCR were performed at days 4, 7 and 11. Nine adult beagle dogs were used to extract the left and right maxillary lateral incisor in vivo forming buccal bone defects. Dogs were randomly divided into 3 groups:blood clots group, PRF group and bone tissue engineering group. Evaluating buccal bone height in each group and the extraction socket bone density were performed at four time points: immediately after surgery, 6 week and 12 weeks later respectively. Results: In vitro, the PRF increased the BMSCs proliferation and significantly improve the ability of osteogenic differentiation and calcification(P<0.05). In animal experiments, PRF showed early healing wound ability. On repairing bone defects, at 6 weeks in the tissue engineering group, the bone hight [(2.63 ±0.57) mm] and bone mineral density [(765.19±59.70) HU] was significantly increased (P<0.05), which compared with blood clots group [(5.65± 2.91) mm, (599.08 ±53.88) HU] and PRF group [(4.14 ±1.16) mm, (644.76 ±65.39) HU]. Conclusion: Cooperative of PRF and BMSCs can effectively repair mandible alveolar bone defects in canine model.