赣南医学院学报
贛南醫學院學報
공남의학원학보
JOURNAL OF GANNAN MEDICAL COLLEGE
2014年
4期
491-493
,共3页
曾祥云%袁思奇%刘琳琳%谭述香%谢招莲%黄彬红%高艳芳
曾祥雲%袁思奇%劉琳琳%譚述香%謝招蓮%黃彬紅%高豔芳
증상운%원사기%류림림%담술향%사초련%황빈홍%고염방
特发性眼眶炎性假瘤%肥大细胞%成纤维细胞
特髮性眼眶炎性假瘤%肥大細胞%成纖維細胞
특발성안광염성가류%비대세포%성섬유세포
Idiopathic Orbital Inflammatory Pseudotumor%mastocyte%fibroblast
目的:探讨肥大细胞在特发性眼眶炎性假瘤纤维化中的作用。方法:将分离纯化的大鼠骨髓肥大细胞和同源大鼠眼眶成纤维细胞,分别进行单独和共育培养。观察肥大细胞和成纤维细胞共育对成纤维细胞在形态和数量上的影响。结果:肥大细胞和成纤维细胞共育能够显著增强成纤维细胞的增殖活性,共育组中单位面积内的成纤维细胞数较对照组显著增加,差异具有统计学意义(P ﹤0.01)。结论:肥大细胞对眼眶成纤维细胞的增殖具有显著的促进作用,提示其在特发性眼眶炎性假瘤纤维化过程中发挥着重要作用。
目的:探討肥大細胞在特髮性眼眶炎性假瘤纖維化中的作用。方法:將分離純化的大鼠骨髓肥大細胞和同源大鼠眼眶成纖維細胞,分彆進行單獨和共育培養。觀察肥大細胞和成纖維細胞共育對成纖維細胞在形態和數量上的影響。結果:肥大細胞和成纖維細胞共育能夠顯著增彊成纖維細胞的增殖活性,共育組中單位麵積內的成纖維細胞數較對照組顯著增加,差異具有統計學意義(P ﹤0.01)。結論:肥大細胞對眼眶成纖維細胞的增殖具有顯著的促進作用,提示其在特髮性眼眶炎性假瘤纖維化過程中髮揮著重要作用。
목적:탐토비대세포재특발성안광염성가류섬유화중적작용。방법:장분리순화적대서골수비대세포화동원대서안광성섬유세포,분별진행단독화공육배양。관찰비대세포화성섬유세포공육대성섬유세포재형태화수량상적영향。결과:비대세포화성섬유세포공육능구현저증강성섬유세포적증식활성,공육조중단위면적내적성섬유세포수교대조조현저증가,차이구유통계학의의(P ﹤0.01)。결론:비대세포대안광성섬유세포적증식구유현저적촉진작용,제시기재특발성안광염성가류섬유화과정중발휘착중요작용。
Objective:To investigate the role of mastocyte(MC)in the proliferation of fibroblast in Idiopathic Orbital In-flammatory Pseudotumor(IOIP). Methods:Rat bone marrow mastocyte and fibroblast were separated and purified,and then cultured them together and respectively. Cell morphology and cell number were observed and analyzed to investigate the role of MC in the proliferation of fibroblast. Results:Proliferation of fibroblast was significantly enhanced by culturing together with MC and cell number of MC was also significantly increased during compound culturing. There was statisti-cally significant difference(P ﹤ 0. 01). Conclusion:MC significantly promotes the proliferation of fibroblast and plays a crucial role during fibrillation in IOIP.