CAJ | 학술논문

为研究利用微生物方法解决养殖水体中氨氮污染的问题，从水产养殖环境中分离出31株氨氮去除菌，并从中筛选出具有较强氨氮去除能力的菌株AD-8，对其脱氮能力进行测定。结果表明，在培养基中氨氮浓度高达200 mg/L时，AD-8菌株对其去除率均达86％以上，15℃下，48 h AD-8对氨氮的去除率可以达到91．2％；在模拟养殖水体中，AD-8氨氮去除率也可达90％。通过形态学特性、生理生化鉴定及16 S rDNA序列分析，菌株AD-8初步鉴定为嗜碱假单胞菌（ Pseudomonas alcaliphila）。
위연구이용미생물방법해결양식수체중안담오염적문제，종수산양식배경중분리출31주안담거제균，병종중사선출구유교강안담거제능력적균주AD-8，대기탈담능력진행측정。결과표명，재배양기중안담농도고체200 mg/L시，AD-8균주대기거제솔균체86％이상，15℃하，48 h AD-8대안담적거제솔가이체도91．2％；재모의양식수체중，AD-8안담거제솔야가체90％。통과형태학특성、생리생화감정급16 S rDNA서렬분석，균주AD-8초보감정위기감가단포균（ Pseudomonas alcaliphila）。
Microbial method was used to remove ammonia-nitrogen pollutant from the aquaculture water ,isola-ted 31 strains with high Ammonia-nitrogen removing ability from the aquaculture sediment .Among these strains , strain AD-8 showed the highest ability of removing ammonia-nitrogen.In the culture,its removal rate to 200 mg/L ammonia nitrogen was 86%,its removal rate was 91.2%in 48 h in low temperature(15℃).In simulated aquacul-ture waste water ,its net removal efficiency of ammonia nitrogen was 90% after 48 hours.AD-8 was identified as Pseudomonas alcaliphila according to its morphological , physiological and biochemical properties and phylogenetic analysis of its 16 S rDNA sequence .