华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
4期
186-191
,共6页
王海娟%包玉英%牛天心%李秀芳
王海娟%包玉英%牛天心%李秀芳
왕해연%포옥영%우천심%리수방
丛枝菌根真菌%有机肥%无机肥%排土场土壤%紫花苜蓿
叢枝菌根真菌%有機肥%無機肥%排土場土壤%紫花苜蓿
총지균근진균%유궤비%무궤비%배토장토양%자화목숙
Arbuscular mycorrhizal fungi%Organic fertilizer%Inorganic fertilizer%Reclamation mine soil%Alfalfa
为解决露天煤矿排土场复垦土壤植被恢复困难的问题，探索有效地改良土壤的措施，采用盆栽试验方法测定排土场土壤中菌剂与肥料配施对紫花苜蓿的生长发育的影响，进而探讨菌根剂与肥料的配施对矿区土壤的改良作用。结果表明：菌根剂与肥料配合施用能显著提高紫花苜蓿的株高、生物量、叶绿素、蛋白质、可溶性糖的含量以及抗氧化酶同工酶活性等，土壤中速效磷、速效氮、有机质、全氮、全磷含量以及土壤微生物数量也都有明显提高。其中尤以有机肥＋无机肥＋菌剂配施效果最为显著，与对照相比，株高、鲜质量、干质量分别增加47％，277％，280％，叶绿素、蛋白质、可溶性糖含量分别增加36％，183％，59％，过氧化氢酶、过氧化物酶、超氧化物歧化酶活性分别增加281％，1250％，15％，土壤速效N、速效P含量分别增加130％，217％，有机质含量增加76％，全N、全P含量分别增加102％，160％，土壤细菌、放线菌、真菌数量分别增加103％，68％，20％。总之，有机肥＋无机肥＋菌剂配施显著促进了紫花苜蓿的生长，提高了排土场土壤的养分含量，是矿区复垦土壤植被恢复中比较好的组合方式。
為解決露天煤礦排土場複墾土壤植被恢複睏難的問題，探索有效地改良土壤的措施，採用盆栽試驗方法測定排土場土壤中菌劑與肥料配施對紫花苜蓿的生長髮育的影響，進而探討菌根劑與肥料的配施對礦區土壤的改良作用。結果錶明：菌根劑與肥料配閤施用能顯著提高紫花苜蓿的株高、生物量、葉綠素、蛋白質、可溶性糖的含量以及抗氧化酶同工酶活性等，土壤中速效燐、速效氮、有機質、全氮、全燐含量以及土壤微生物數量也都有明顯提高。其中尤以有機肥＋無機肥＋菌劑配施效果最為顯著，與對照相比，株高、鮮質量、榦質量分彆增加47％，277％，280％，葉綠素、蛋白質、可溶性糖含量分彆增加36％，183％，59％，過氧化氫酶、過氧化物酶、超氧化物歧化酶活性分彆增加281％，1250％，15％，土壤速效N、速效P含量分彆增加130％，217％，有機質含量增加76％，全N、全P含量分彆增加102％，160％，土壤細菌、放線菌、真菌數量分彆增加103％，68％，20％。總之，有機肥＋無機肥＋菌劑配施顯著促進瞭紫花苜蓿的生長，提高瞭排土場土壤的養分含量，是礦區複墾土壤植被恢複中比較好的組閤方式。
위해결로천매광배토장복은토양식피회복곤난적문제，탐색유효지개량토양적조시，채용분재시험방법측정배토장토양중균제여비료배시대자화목숙적생장발육적영향，진이탐토균근제여비료적배시대광구토양적개량작용。결과표명：균근제여비료배합시용능현저제고자화목숙적주고、생물량、협록소、단백질、가용성당적함량이급항양화매동공매활성등，토양중속효린、속효담、유궤질、전담、전린함량이급토양미생물수량야도유명현제고。기중우이유궤비＋무궤비＋균제배시효과최위현저，여대조상비，주고、선질량、간질량분별증가47％，277％，280％，협록소、단백질、가용성당함량분별증가36％，183％，59％，과양화경매、과양화물매、초양화물기화매활성분별증가281％，1250％，15％，토양속효N、속효P함량분별증가130％，217％，유궤질함량증가76％，전N、전P함량분별증가102％，160％，토양세균、방선균、진균수량분별증가103％，68％，20％。총지，유궤비＋무궤비＋균제배시현저촉진료자화목숙적생장，제고료배토장토양적양분함량，시광구복은토양식피회복중비교호적조합방식。
Opencast coal mining leaves large areas of refuse dump ,because of the environment pollution ,soil e-rosion、nutrient-poor of soil and biodiversity loss , vegetation reclamation in these areas is difficult , so explore the measures for soil improvement is important to practice .In this paper ,the effect of combined application of microbial inoculum and fertilizer on growth of alfalfa in refuse dump through pot culture experiment , and then explore the effect of combined application of microbial inoculum and fertilizer on improvement of mine soil .The results showed that combined application of microbial inoculum and fertilizer significantly increased the alfalfa ′s biomass , plant height,the content of chlorophyll ,protein,soluble sugar and activity of antioxidant enzyme isozyme ,nutrient content of reclaimed soil was also significantly increased .The most effective treatments was organic fertilizer +inorganic fer-tilizer+microbial inoculum applied ,compared with the control ,the increasing rates of plant height ,fresh weight and dry weight reached 47%,277%and 280%,the increasing rates of chlorophyll content ,protein content and soluble sugar content reached 36%,183%and 59 %,the increased rates of hydrogen peroxide activity ,peroxidase activity and superoxide dismutase activity reached 281%,1250% and 15%,the increased rates of soil available N ,P,or-ganic matter,total N and P reached 130%,217%,76%,102% and 160%,the increased rates of bacteria ,actino-mycetes and fungi reached 103%,68%and 20%.In general,combined application of microbial inoculum ,inorganic fertilizer and organic fertilizer significantly promoted the growth of alfalfa and improved the fertility of dump soil ,was a good combination of vegetation reclamation in mine wasteland soil .
