CAJ | 학술논문

为探讨壳寡糖对菜薹接种立枯丝核菌后的发病率、病情相关蛋白和内源激素含量的影响。以菜心品种油青四九为材料，于菜薹形成初期叶面喷施0．4，2，8 mg/L壳寡糖预处理2 d，以喷施无菌水为对照，灌根接种立枯丝核菌。结果表明：0．4 mg/L壳寡糖处理可提高菜薹植株对立枯丝核菌的抗性，降低发病率，减少由于病害造成的产量下降。与单独接种立枯丝核菌相比，接种6 d内，0．4 mg/L壳寡糖处理可进一步增强病原菌对菜薹植株叶片中几丁质酶、β-1，3葡聚糖酶的诱导作用；接种6 d后，则显著促进内源激素吲哚乙酸（ IAA）和脱落酸（ ABA）的形成；接种处理期间，0．4 mg/L壳寡糖处理均显著诱导了乙烯的形成。表明，接种早期，适宜浓度的壳寡糖可提高植株体内病情相关蛋白几丁质酶和β-1，3葡聚糖酶的活性，进而通过较高水平的内源激素乙烯、IAA和ABA的代谢传递抗病信号，从而诱导菜薹植株对立枯病的系统抗性。
위탐토각과당대채대접충립고사핵균후적발병솔、병정상관단백화내원격소함량적영향。이채심품충유청사구위재료，우채대형성초기협면분시0．4，2，8 mg/L각과당예처리2 d，이분시무균수위대조，관근접충립고사핵균。결과표명：0．4 mg/L각과당처리가제고채대식주대립고사핵균적항성，강저발병솔，감소유우병해조성적산량하강。여단독접충립고사핵균상비，접충6 d내，0．4 mg/L각과당처리가진일보증강병원균대채대식주협편중궤정질매、β-1，3포취당매적유도작용；접충6 d후，칙현저촉진내원격소신타을산（ IAA）화탈락산（ ABA）적형성；접충처리기간，0．4 mg/L각과당처리균현저유도료을희적형성。표명，접충조기，괄의농도적각과당가제고식주체내병정상관단백궤정질매화β-1，3포취당매적활성，진이통과교고수평적내원격소을희、IAA화ABA적대사전체항병신호，종이유도채대식주대립고병적계통항성。
The aim of our study was to investigate the influences of chitooligosaccharide on sheath blight occur -rence,pathogenesis-related proteins(PRs) and endogenous hormones contents .Youqingsijiu flowering Chinese cab-bage(Brassica campestris L.ssp.chinensis var.utilis Tsen et Lee) was chosen as the material .At the early stage of stalk formation,0.4,2 and 8 mg/L chitooligosaccharide and sterile water (control) were sprayed,and 2 days later, plants were inoculated by Rhizoctonia solani.The results showed that 0.4 mg/L chitooligosaccharide could increase the resistance of plants to R.solani,reduce the incidence of sheath blight ,and thus reduce the loss of the yield caused with R.solani.Compared with inoculation alone ,foliar application of 0.4 mg/L chitooligosaccharide could further enhanced the activities of chitinase and-1,3 glucanase within 6 days of inoculation,and the production of ethylene ,indole acetic acid and abscisic acid after 6 days of inoculation .These data suggested that it is important for inducing systematic acquired resistance of flowering Chinese cabbage to sheath blight to induce high level of PRs in a shorter time after inoculation ,and later keep high contents of endogenous hormones .