类猪圆环病毒 P1转录的 Northern Blot 分析
류저원배병독 P1전록적 Northern Blot 분석
Transcriptional Analysis of Porcine Circovirus-like Virus P1 by Northern Blot
  • 万方数据
    华北农学报 화북농학보
    ACTA AGRICULTURAE BOREALI-SINICA
    2014年 4期 160-163 ,共4页

    温立斌%王凤芝%何孔旺%倪艳秀%张雪寒%李彬%王小敏%郭容利%周俊明%俞正玉%茅爱华%吕立新%解建平

    온립빈%왕봉지%하공왕%예염수%장설한%리빈%왕소민%곽용리%주준명%유정옥%모애화%려립신%해건평

    类猪圆环病毒P1%转录分析%Northern Blot 類豬圓環病毒P1%轉錄分析%Northern Blot
    류저원배병독P1%전록분석%Northern Blot
    Porcine circovirus-like virus P1%Transcriptional analysis%Northern Blot
    为鉴定类猪圆环病毒P1的转录本，采用地高辛标记的RNA探针，通过Northern Blot方法，对转染PK15细胞的类猪圆环病毒P1进行了RNA转录分析。结果表明，Northern杂交可检测到2个RNA转录本，包括正链上ORF3的RNA和负链上ORF1的RNA。同时，ORF3 RNA表达时间比ORF1的短，且丰度也大大低于ORF1。类猪圆环病毒P1至少存在ORF1和ORF32个转录本。
    위감정류저원배병독P1적전록본，채용지고신표기적RNA탐침，통과Northern Blot방법，대전염PK15세포적류저원배병독P1진행료RNA전록분석。결과표명，Northern잡교가검측도2개RNA전록본，포괄정련상ORF3적RNA화부련상ORF1적RNA。동시，ORF3 RNA표체시간비ORF1적단，차봉도야대대저우ORF1。류저원배병독P1지소존재ORF1화ORF32개전록본。
    The purpose of this study is to identify transcripts of porcine circovirus-like virus P1.In this work, the RNAs of porcine circovirus-like virus P1 synthesized in PK15 cells were characterized by Northern Blot analysis using the DIG-labeled riboprobes .The results showed two RNAs were detected .They included ORF3 RNA and ORF1 RNA.Meanwhile,ORF1 RNA had both more abundant and longer expression than those of ORF3 RNA. Northern Blot analysis indicated that at least two viral-specific RNAs were synthesized in P 1.infected PK15 cells.
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