CAJ | 학술논문

为了明确辣椒花青素生物合成途径中相关基因的表达调控模式，选取3种不同果实颜色的辣椒品系（紫晶、淡紫、水晶）为试材，提取果实不同发育阶段的RNA并反转录成cDNA，利用qRT-PCR技术分析相关基因（ CHS、CHI、F3H、F3′5′H、DFR、ANS、UFGT、ANP、GST）在不同辣椒材料果实不同发育阶段的表达情况。结果显示：CHS、CHI在3种材料果实发育过程中表达量都很少且无规律可循，F3H在淡紫、紫晶中表达量较高，且在果实发育的第15～20天时出现较大峰值，与之相比在水晶中的表达量可以忽略不计。 F3′5′H、ANP、GST、DFR、UFGT在3种材料中相对表达量随着果实的发育分别呈现同步变化，且F3′5′H、ANP、GST在材料紫晶果实发育的第15天相对表达量出现较大峰值，ANS在3种材料果实发育过程中的相对表达量变化没有一定规律可循。 F3H、F3′5′H、ANP、GST是辣椒花青素代谢途径的关键基因。
위료명학랄초화청소생물합성도경중상관기인적표체조공모식，선취3충불동과실안색적랄초품계（자정、담자、수정）위시재，제취과실불동발육계단적RNA병반전록성cDNA，이용qRT-PCR기술분석상관기인（ CHS、CHI、F3H、F3′5′H、DFR、ANS、UFGT、ANP、GST）재불동랄초재료과실불동발육계단적표체정황。결과현시：CHS、CHI재3충재료과실발육과정중표체량도흔소차무규률가순，F3H재담자、자정중표체량교고，차재과실발육적제15～20천시출현교대봉치，여지상비재수정중적표체량가이홀략불계。 F3′5′H、ANP、GST、DFR、UFGT재3충재료중상대표체량수착과실적발육분별정현동보변화，차F3′5′H、ANP、GST재재료자정과실발육적제15천상대표체량출현교대봉치，ANS재3충재료과실발육과정중적상대표체량변화몰유일정규률가순。 F3H、F3′5′H、ANP、GST시랄초화청소대사도경적관건기인。
In order to clarify the expression and regulation patterns of related gene in the process of anthocyanin biosynthesis in pepper ,select three pepper strains which fruit of different color for materials ( Zijing,Danzi,Shui-jing) ,extract RNA of different stages of the fruit and reverse transcribed into cDNA ,then using qRT-PCR technolo-gy to analysis of expression of structure genes ( CHS,CHI,F3H,F3′5′H,DFR,ANS,UFGT,ANP and GST) in pep-per fruit of three kinds of materials in different development stages .The results showed that the expression of CHS, CHI are rarely and disorder in the process of fruit development of three kinds of materials ,the expression of F3H is higher in Danzi and Zijing ,and appear larger peak in the fruit development of 15-20 days,compared with it,the a-mount of expression in Shuijing can be negligible .The relative transcription level of F3′5′H,ANP,GST,DFR and UFGT express synchronous change in three kinds of materials ,and the relative expression of F3′5′H,ANP and GST in the 15 th day of Zijing fruit appeared larger peak ,the relative transcription level of ANS without some rules to fol-low.F3H,F3′5′H,ANP and GST are the key genes to the anthocyanins metabolic pathway in pepper .