华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
4期
71-79
,共9页
张涛%张跟喜%韩昆鹏%王金玉%樊庆灿%李婷婷%段炼%王永娟
張濤%張跟喜%韓昆鵬%王金玉%樊慶燦%李婷婷%段煉%王永娟
장도%장근희%한곤붕%왕금옥%번경찬%리정정%단련%왕영연
京海黄鸡%MyoG%密码子%偏好性%聚类分析
京海黃鷄%MyoG%密碼子%偏好性%聚類分析
경해황계%MyoG%밀마자%편호성%취류분석
Jinghai yellow chicken%MyoG%Codon%Bias%Clustering analysis
为了解鸡MyoG基因的密码子特性，以便于为MyoG基因的表达选择合适的外源表达系统，使用在线工具CUSP和CHIPS以及codonW软件对鸡MyoG基因进行分析，并与鸡基因、模式动物基因组和其他动物MyoG基因进行比较。结果表明，MyoG基因偏好于G/C结尾的密码子，CDS序列中，GC含量大于AT含量。 MyoG基因与鸡其他31个基因密码子偏好性相似，均偏好于G/C结尾的密码子。与其他物种基因组密码子偏好性比较结果显示，密码子偏好性与小鼠差异最小，说明小鼠可以作为MyoG基因外源表达的宿主。与其他动物MyoG基因密码子偏好性比较显示，MyoG基因在包括京海黄鸡在内的32个物种中均有较高表达，几乎所有MyoG基因编码区对GC有较强的偏好性。基于RSCU值的聚类和基于CDS区系统发育树结果显示，亲缘关系近的物种之间倾向于拥有相似的密码子偏好性。
為瞭解鷄MyoG基因的密碼子特性，以便于為MyoG基因的錶達選擇閤適的外源錶達繫統，使用在線工具CUSP和CHIPS以及codonW軟件對鷄MyoG基因進行分析，併與鷄基因、模式動物基因組和其他動物MyoG基因進行比較。結果錶明，MyoG基因偏好于G/C結尾的密碼子，CDS序列中，GC含量大于AT含量。 MyoG基因與鷄其他31箇基因密碼子偏好性相似，均偏好于G/C結尾的密碼子。與其他物種基因組密碼子偏好性比較結果顯示，密碼子偏好性與小鼠差異最小，說明小鼠可以作為MyoG基因外源錶達的宿主。與其他動物MyoG基因密碼子偏好性比較顯示，MyoG基因在包括京海黃鷄在內的32箇物種中均有較高錶達，幾乎所有MyoG基因編碼區對GC有較彊的偏好性。基于RSCU值的聚類和基于CDS區繫統髮育樹結果顯示，親緣關繫近的物種之間傾嚮于擁有相似的密碼子偏好性。
위료해계MyoG기인적밀마자특성，이편우위MyoG기인적표체선택합괄적외원표체계통，사용재선공구CUSP화CHIPS이급codonW연건대계MyoG기인진행분석，병여계기인、모식동물기인조화기타동물MyoG기인진행비교。결과표명，MyoG기인편호우G/C결미적밀마자，CDS서렬중，GC함량대우AT함량。 MyoG기인여계기타31개기인밀마자편호성상사，균편호우G/C결미적밀마자。여기타물충기인조밀마자편호성비교결과현시，밀마자편호성여소서차이최소，설명소서가이작위MyoG기인외원표체적숙주。여기타동물MyoG기인밀마자편호성비교현시，MyoG기인재포괄경해황계재내적32개물충중균유교고표체，궤호소유MyoG기인편마구대GC유교강적편호성。기우RSCU치적취류화기우CDS구계통발육수결과현시，친연관계근적물충지간경향우옹유상사적밀마자편호성。
In order to understand the codon characteristics of MyoG gene in chicken and select the appropriate exogenous expression system , online tools CUSP , CHIPS and codonW software were used to analyze MyoG gene . MyoG gene was compared to other genes of chicken ,model animal genomes and MyoG gene of other species .Result showed that MyoG of chicken was bias toward the synonymous codons with G and C at the third codon position .GC content was greater than AT content in CDS region .MyoG gene and other thirty-one genes of chicken shared similar codon bias toward the synonymous codons with G and C at the third codon position .Codon bias comparison to other species showed that differences of codon bias between MyoG gene and Mus musculus was the minimum,indicating that Mus musculus could be used as exogenous gene expression host of MyoG gene.Codon bias comparison to the MyoG gene of other species indicated that MyoG gene expressed high in all the thirty-two species ,including Jinghai yellow chicken .Almost all the thirty-two MyoG genes were bias toward to GC .Result of clustering based on RSCU value and phylogenetic tree based on CDS region showed that species with close genetic relationship tend to have similar codon bias .
