CAJ | 학술논문

应用DNA重组技术将编码大豆液泡膜Na＋/H＋反向转运蛋白GmNHX1和百脉根液泡膜H＋-PPase LcVP1的开放阅读框片段同时构建在pCambia1301载体中，形成p1301-GmNHX1-LcVP1双价耐盐基因植物表达载体。以Co-ker 312棉花品种的胚性愈伤组织为外植体，采用根癌农杆菌介导法将其转入棉花中，并利用潮霉素筛选获得抗性植株。对抗性植株的T1幼苗进行分子和生理指标检测，表明外源基因已成功整合到棉花基因组中，并不同程度地提高了转基因株系耐盐能力。
응용DNA중조기술장편마대두액포막Na＋/H＋반향전운단백GmNHX1화백맥근액포막H＋-PPase LcVP1적개방열독광편단동시구건재pCambia1301재체중，형성p1301-GmNHX1-LcVP1쌍개내염기인식물표체재체。이Co-ker 312면화품충적배성유상조직위외식체，채용근암농간균개도법장기전입면화중，병이용조매소사선획득항성식주。대항성식주적T1유묘진행분자화생리지표검측，표명외원기인이성공정합도면화기인조중，병불동정도지제고료전기인주계내염능력。
By use of DNA recombination method ,a vector,p1301-GmNHX1-LcVP1,harboring two open reading frames of soybean tonoplast Na+/H+antiporter GmNHX1 and Lotus corniculatus H+-PPase LcVP1,was constructed through sequential restriction digests and ligations recombination .The two genes driven by CaMV 35 S promoters were suc-cessfully transferred into cotton cultivars Coker 312 via Agrobacterium with hygromycin selection system .The molec-ular and physiological analysises of T 1 generation indicated that genes were integrated into the cotton genome and enhanced transgenic cotton salt tolerance .