CAJ | 학술논문

在粳稻中花11的组培后代中发现1个突变体ep7，其稻穗直立，且株高、有效穗、粒长和千粒质量等均显著降低；利用该突变体与籼稻华粳籼74构建F2分离群体对突变体进行遗传分析，结果表明，该突变表型由1对核隐性基因控制，并用分子标记将该基因定位于第7染色体长臂上，命名为EP7，其与InDel标记ID5198-2和ID4309-1的遗传距离分别为0．7 cM 和0．5 cM；生物信息学和序列多态性分析表明，可能是大片段的DNA插入造成候选基因功能缺失，导致该突变表型的产生。
재갱도중화11적조배후대중발현1개돌변체ep7，기도수직립，차주고、유효수、립장화천립질량등균현저강저；이용해돌변체여선도화갱선74구건F2분리군체대돌변체진행유전분석，결과표명，해돌변표형유1대핵은성기인공제，병용분자표기장해기인정위우제7염색체장비상，명명위EP7，기여InDel표기ID5198-2화ID4309-1적유전거리분별위0．7 cM 화0．5 cM；생물신식학화서렬다태성분석표명，가능시대편단적DNA삽입조성후선기인공능결실，도치해돌변표형적산생。
A rice mutant erect panicle 7(ep7),obtained from tissue culture of a japonica variety Zhonghua 11, showed a dense and erect panicle and significantly reduced plant height ,effective panicle number per plant ,grain length and 1000-grain weight.The genetic analysis of a F2 segregation population derived from the cross between ep7 and an indica variety Huajingxian 74 demonstrated that the mutant phenotype was controlled by a single recessive gene on the long arm of chromosome 7,termed EP7,between two InDel markers ID5198-2 and ID4309-1 with 0.7 cM and 0.5 cM genetic distance,respectively.Morever,bioinformatic analysis and sequence polymorphorism re-vealed that the mutant phenotype might be caused by a large DNA fragment insertion in EP7 gene.