华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
4期
37-43
,共7页
陈涛%刘燕清%张亚东%朱镇%赵庆勇%周丽慧%姚姝%于新%赵凌%王才林
陳濤%劉燕清%張亞東%硃鎮%趙慶勇%週麗慧%姚姝%于新%趙凌%王纔林
진도%류연청%장아동%주진%조경용%주려혜%요주%우신%조릉%왕재림
水稻%窄卷叶%突变体%遗传分析%基因定位
水稻%窄捲葉%突變體%遺傳分析%基因定位
수도%착권협%돌변체%유전분석%기인정위
Oryza sativa L.%Narrow and rolled leaf%Mutant%Genetic analysis%Gene mapping
为发掘、定位和克隆水稻窄卷叶相关突变基因，揭示叶片发育机理。对武运粳7号条纹叶枯病抗性改良品系中发现的窄卷叶突变体进行了表型观察、遗传分析及基因定位。结果表明，与野生型相比，突变体在叶片形态发生改变的同时，其株高、分蘖性、穗长、枝梗数、每穗实粒数、穗粒数、结实率、千粒质量等农艺性状也存在显著差异。遗传分析表明，该突变性状受一对隐性基因控制。以500株辐恢838/nrl（t）的F2隐性单株为定位群体，利用SSR标记将NRL（t）基因定位于水稻第11染色体短臂末端RM11-01、RM11-11之间，物理距离约为160 kb的范围内。通过水稻基因组注释数据库分析发现，在该区域存在26个预测的基因，测序分析表明已报道的homeobox3A基因可能是潜在的候选基因，这为进一步研究该基因奠定了基础。
為髮掘、定位和剋隆水稻窄捲葉相關突變基因，揭示葉片髮育機理。對武運粳7號條紋葉枯病抗性改良品繫中髮現的窄捲葉突變體進行瞭錶型觀察、遺傳分析及基因定位。結果錶明，與野生型相比，突變體在葉片形態髮生改變的同時，其株高、分蘗性、穗長、枝梗數、每穗實粒數、穗粒數、結實率、韆粒質量等農藝性狀也存在顯著差異。遺傳分析錶明，該突變性狀受一對隱性基因控製。以500株輻恢838/nrl（t）的F2隱性單株為定位群體，利用SSR標記將NRL（t）基因定位于水稻第11染色體短臂末耑RM11-01、RM11-11之間，物理距離約為160 kb的範圍內。通過水稻基因組註釋數據庫分析髮現，在該區域存在26箇預測的基因，測序分析錶明已報道的homeobox3A基因可能是潛在的候選基因，這為進一步研究該基因奠定瞭基礎。
위발굴、정위화극륭수도착권협상관돌변기인，게시협편발육궤리。대무운갱7호조문협고병항성개량품계중발현적착권협돌변체진행료표형관찰、유전분석급기인정위。결과표명，여야생형상비，돌변체재협편형태발생개변적동시，기주고、분얼성、수장、지경수、매수실립수、수립수、결실솔、천립질량등농예성상야존재현저차이。유전분석표명，해돌변성상수일대은성기인공제。이500주복회838/nrl（t）적F2은성단주위정위군체，이용SSR표기장NRL（t）기인정위우수도제11염색체단비말단RM11-01、RM11-11지간，물리거리약위160 kb적범위내。통과수도기인조주석수거고분석발현，재해구역존재26개예측적기인，측서분석표명이보도적homeobox3A기인가능시잠재적후선기인，저위진일보연구해기인전정료기출。
Leaf morphology is one of key components for rice plant architecture , so excavation , mapping and cloning related mutant genes are very important for revealing mechanism of leaf development and breeding new rice varieties with ideotype and super-high yield.In this study,the narrow and rolled mutant ntl (t) was found from the im-proved line of rice stripe disease for Wuyunjing No .7 and the mutant phenotype genetic analysis and gene mapping was researched .Compared with wild type , the mutant showed multipe abnormal phenotypes , such as shorter leaf length and width,rolled leaf,dwarfism,more tillers,reduced panicle length,branch number,filled grain number per spike ,spikelets per spike , seed-setting rate and 1000-grain weight .Genetic analysis indicated that the mutant was controlled by a single recessive gene .Using 500 recessive mutant individuals in the F 2 population derived from the cross between Fuhui 838 and nrl(t) ,the gene NRL(t) was located to a confined region of 160 kb flanked by two mic-rosatellite markers RM11-01 and RM11-11 on the short arm of chromosome 11.Based on the annotation result of rice genome,26 open reading frames(ORFs)were predicted in this region.Sequencing analysis showed that the ho-meobox3 A gene reported might be the potential candidate gene .These results are very valuable for further study on this gene .
