CAJ | 학술논문

为了发掘小麦育性基因，采用同源克隆的方法，从小麦花药中克隆了3个与拟南芥AtMS2和水稻OsMS2基因同源的cDNA序列。TaMSR-1、TaMSR-2和TaMSR-3分别具有1842，1824，1830 bp 的开放阅读框，各自编码613，607，609个氨基酸。推导的蛋白质序列中包含2个保守区：NAD_binding 和Sterile保守功能域。氨基酸序列分析发现，TaMSR与水稻OsMSR2和拟南芥AtMSR2具有较高的相似性。采用基因特异定位引物PCR技术对中国春缺失系材料进行扩增，将TaMSR-1、TaMSR-2和TaMSR-3基因分别定位于4AS、4DL和4BL染色体上。半定量RT-PCR分析表明，TaMSR基因是花药组织特异表达的基因。这些结果说明，TaMSR基因可能在小麦花药发育过程中起重要作用。
위료발굴소맥육성기인，채용동원극륭적방법，종소맥화약중극륭료3개여의남개AtMS2화수도OsMS2기인동원적cDNA서렬。TaMSR-1、TaMSR-2화TaMSR-3분별구유1842，1824，1830 bp 적개방열독광，각자편마613，607，609개안기산。추도적단백질서렬중포함2개보수구：NAD_binding 화Sterile보수공능역。안기산서렬분석발현，TaMSR여수도OsMSR2화의남개AtMSR2구유교고적상사성。채용기인특이정위인물PCR기술대중국춘결실계재료진행확증，장TaMSR-1、TaMSR-2화TaMSR-3기인분별정위우4AS、4DL화4BL염색체상。반정량RT-PCR분석표명，TaMSR기인시화약조직특이표체적기인。저사결과설명，TaMSR기인가능재소맥화약발육과정중기중요작용。
In search for fertility gene in wheat , three cDNAs that homologous to AtMS2 and putative OsMS2 , designated as TaMSR-1 ,TaMSR-2 and TaMSR-3 were cloned from wheat anther by homologogy-based cloning ap-proach .TaMSR-1 ,TaMSR-2 and TaMSR-3 contain open reading frames of 1 842 ,1 824 ,1 830 bp encoding proteins of 613 ,607 ,609 amino acid residues including two male sterile conserved domains:NAD-binding domain and male sterile C-terminal domain , respectively .The deduced amino acid sequence of TaMSR had high similarities with OsMSR2 and AtMSR2.Using Chinese_Spring_Deletion line,TaMSR-1,TaMSR-2 and TaMSR-3 genes were located on chromosome 4AS,4DL and 4BL,respectively.Semi-quantitative RT-PCR analysis revealed that TaMSR gene was anther specific expressed gene .These results suggested that TaMSR may plays an important role in wheat anther de-velopment .