CAJ | 학술논문

以Y系山定子为中间砧嫁接的苹果树当年开始花芽形态分化，而未用中间砧的苹果树则未形成花芽。分析了中间砧Y系山定子与基砧海棠不同组织中花发育相关基因MADS5、MADS2、MdFT2、MdAFL1、MdAFL2、MdTFL1的表达特征。结果表明，MADS5基因的表达模式及表达量是影响苹果花芽发育的一个重要因素；将山定子中的MADS5基因在拟南芥中过量表达，转基因拟南芥表现出早花表型，比对照早开花7～10 d；相同条件下转MdFT2基因的拟南芥比对照早开花4～7 d。认为在利用基因工程改良苹果早花发育的研究中，MADS5基因更具有应用潜力。
이Y계산정자위중간침가접적평과수당년개시화아형태분화，이미용중간침적평과수칙미형성화아。분석료중간침Y계산정자여기침해당불동조직중화발육상관기인MADS5、MADS2、MdFT2、MdAFL1、MdAFL2、MdTFL1적표체특정。결과표명，MADS5기인적표체모식급표체량시영향평과화아발육적일개중요인소；장산정자중적MADS5기인재의남개중과량표체，전기인의남개표현출조화표형，비대조조개화7～10 d；상동조건하전MdFT2기인적의남개비대조조개화4～7 d。인위재이용기인공정개량평과조화발육적연구중，MADS5기인경구유응용잠력。
The flower bud formation of apple trees grafted into Y series dwarf apple interstock was earlier than those not grafted into the Y series interstock .In this study ,we analyzed the expression pattern of several flower de-velopment genes,including MADS5,MADS2,MdFT2,MdAFL1,MdAFL2,MdTFL1, in the different tissues of Y se-ries dwarf apple interstock ( Malus baccata ) and rootstock ( Malus asiatica ) .The results showed that MADS5 gene was a key regulator in control of apple bud formation due to its special expression pattern and higher expression lev -el in bud and sepal tissues .Overexpression of Y series dwarf apple interstock MADS5 gene in Arabidopsis reduced the vegetative growth and generation time and led to an early blooming phenotype .Transgenic plants overexpression MADS5 gene blossomed earlier about 7-10 days compared with the wild type plants .Meanwhile ,overexpression of MdFT2 gene in Arabidopsis also promoted flowering average 4-7 days earlier compared with the wild type plants .It is concluded that MADS5 gene is more useful than MdFT2 gene in the study of early blooming promoting and fruit-ing of apple plants through genetic engineering methods .