CAJ | 학술논문

为了探讨转录因子在小麦抗旱分子机制中的作用，利用Affymetrix小麦表达谱芯片对渗透胁迫下普通小麦（石麦15）根系和叶片诱导表达谱进行了分析。在表达谱芯片上筛选到1个渗透胁迫诱导表达的MYB转录因子EST序列（ GenBank 登录号：BJ264503）。根据该EST序列设计引物筛选石麦15基因组BAC文库，获得1个含有该EST序列的BAC单克隆。以BAC单克隆质粒为模板，通过BAC测序克隆了小麦MYB转录因子基因（ TaMYBSM151）及其5′侧翼序列。 TaMYBSM151的开放读码框长936 bp，编码1个含有311个氨基酸的R1-MYB转录因子。 TaMYBSM151基因包含3个外显子和2个内含子。在TaMYBSM1515′侧翼序列中，预测出33个脱水响应相关的顺式作用元件。实时定量PCR结果表明， TaMYBSM151受渗透胁迫诱导表达，但在根和叶中表达模式完全不同。以上结果表明， TaMYBSM151可能为脱水响应基因。
위료탐토전록인자재소맥항한분자궤제중적작용，이용Affymetrix소맥표체보심편대삼투협박하보통소맥（석맥15）근계화협편유도표체보진행료분석。재표체보심편상사선도1개삼투협박유도표체적MYB전록인자EST서렬（ GenBank 등록호：BJ264503）。근거해EST서렬설계인물사선석맥15기인조BAC문고，획득1개함유해EST서렬적BAC단극륭。이BAC단극륭질립위모판，통과BAC측서극륭료소맥MYB전록인자기인（ TaMYBSM151）급기5′측익서렬。 TaMYBSM151적개방독마광장936 bp，편마1개함유311개안기산적R1-MYB전록인자。 TaMYBSM151기인포함3개외현자화2개내함자。재TaMYBSM1515′측익서렬중，예측출33개탈수향응상관적순식작용원건。실시정량PCR결과표명， TaMYBSM151수삼투협박유도표체，단재근화협중표체모식완전불동。이상결과표명， TaMYBSM151가능위탈수향응기인。
In order to determine the role of transcriptional factors in drought-resistant molecular mechanism of common wheat ,Affymetrix GeneChip Wheat Genome Array was used to analyze the expression-profile of common wheat(Shimai 15)root and leaf under osmotic stress.An osmotic-stress induced EST sequence was identified (Gen-Bank accession No .BJ264503 ) in the expression-profile chip .One BAC clone was isolated from Shimai 15 genome BAC library using primer pairs designed based on EST-BJ264503 sequence .The MYB transcriptional factor gene ( TaMYBSM151 ) and its 5′-flanking sequence were cloned by sequencing walking using BAC clone plasmid as tem-plate.The open reading frame of TaMYBSM151 is 936 bp long coding for a putative 311-amino-acid R1-MYB tran-scriptional factor .TaMYBSM151 contained three exons and two introns .In 5′-flanking sequence of TaMYBSM151 , 33 dehydration-response cis-elements were predicted .Real-time RT-PCR revealed that the expression of TaMYB-SM151 was induced by osmotic stress ,but showed different expression patterns .The results above indicated TaMYB-SM151 may be a dehydration-response gene .