华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
4期
13-18
,共6页
郭秀林%刘子会%赵会嶶%徐婧%李慧聪
郭秀林%劉子會%趙會嶶%徐婧%李慧聰
곽수림%류자회%조회미%서청%리혜총
冬小麦%热激蛋白%抗氧化酶%基因表达%获得耐热性
鼕小麥%熱激蛋白%抗氧化酶%基因錶達%穫得耐熱性
동소맥%열격단백%항양화매%기인표체%획득내열성
Winter wheat%Hsp%Antioxidant enzymes%Gene expression%Acquired heat-tolerance
小麦经过适当高温锻炼可产生获得耐热性,为揭示其分子生物学机理,选取耐热品种邯6172和热敏感品种石新733,对34℃热锻炼和42℃高温胁迫条件下叶片2个热激蛋白基因和6个抗氧化酶基因转录表达进行了荧光定量分析,并测定了热致死时间。结果显示,34℃热锻炼可不同程度地提高耐热品种和热敏感品种叶片的热致死时间,42℃高温胁迫下耐热品种表现出更强耐热性。34℃热锻炼可诱导耐热品种热激蛋白TaHsp70和TaHsp16.9基因在不同时间点出现不同程度表达高峰,植株遭受高温胁迫时基因表达上调幅度更强于热敏感品种。34℃热锻炼,耐热品种和热敏感品种Ta( Cu/Zn) SOD、TaMnSOD、TaApx1、TaApx4和TaApx5的表达均呈现间歇性升高趋势,耐热品种的表达量高于热敏感品种,遭受高温胁迫时基因表达持续上升的幅度相对更大。比较出现峰值的时间,热激蛋白和抗氧化酶基因的表达均不晚于热致死时间。说明,热锻炼可诱导热激蛋白和相关酶类基因上调表达,共同调控小麦获得耐热性。不同小麦品种获得耐热性的程度和持续的时间均不同,热锻炼后获得耐热性强度越大,保持的时间越长,遭遇高温胁迫时耐热性越强。
小麥經過適噹高溫鍛煉可產生穫得耐熱性,為揭示其分子生物學機理,選取耐熱品種邯6172和熱敏感品種石新733,對34℃熱鍛煉和42℃高溫脅迫條件下葉片2箇熱激蛋白基因和6箇抗氧化酶基因轉錄錶達進行瞭熒光定量分析,併測定瞭熱緻死時間。結果顯示,34℃熱鍛煉可不同程度地提高耐熱品種和熱敏感品種葉片的熱緻死時間,42℃高溫脅迫下耐熱品種錶現齣更彊耐熱性。34℃熱鍛煉可誘導耐熱品種熱激蛋白TaHsp70和TaHsp16.9基因在不同時間點齣現不同程度錶達高峰,植株遭受高溫脅迫時基因錶達上調幅度更彊于熱敏感品種。34℃熱鍛煉,耐熱品種和熱敏感品種Ta( Cu/Zn) SOD、TaMnSOD、TaApx1、TaApx4和TaApx5的錶達均呈現間歇性升高趨勢,耐熱品種的錶達量高于熱敏感品種,遭受高溫脅迫時基因錶達持續上升的幅度相對更大。比較齣現峰值的時間,熱激蛋白和抗氧化酶基因的錶達均不晚于熱緻死時間。說明,熱鍛煉可誘導熱激蛋白和相關酶類基因上調錶達,共同調控小麥穫得耐熱性。不同小麥品種穫得耐熱性的程度和持續的時間均不同,熱鍛煉後穫得耐熱性彊度越大,保持的時間越長,遭遇高溫脅迫時耐熱性越彊。
소맥경과괄당고온단련가산생획득내열성,위게시기분자생물학궤리,선취내열품충함6172화열민감품충석신733,대34℃열단련화42℃고온협박조건하협편2개열격단백기인화6개항양화매기인전록표체진행료형광정량분석,병측정료열치사시간。결과현시,34℃열단련가불동정도지제고내열품충화열민감품충협편적열치사시간,42℃고온협박하내열품충표현출경강내열성。34℃열단련가유도내열품충열격단백TaHsp70화TaHsp16.9기인재불동시간점출현불동정도표체고봉,식주조수고온협박시기인표체상조폭도경강우열민감품충。34℃열단련,내열품충화열민감품충Ta( Cu/Zn) SOD、TaMnSOD、TaApx1、TaApx4화TaApx5적표체균정현간헐성승고추세,내열품충적표체량고우열민감품충,조수고온협박시기인표체지속상승적폭도상대경대。비교출현봉치적시간,열격단백화항양화매기인적표체균불만우열치사시간。설명,열단련가유도열격단백화상관매류기인상조표체,공동조공소맥획득내열성。불동소맥품충획득내열성적정도화지속적시간균불동,열단련후획득내열성강도월대,보지적시간월장,조우고온협박시내열성월강。
Wheat seedlings can get heat tolerance after exposed to heat acclimation .In order to study the mo-lecular mechanism ,heat killing times of leaf were measured and genes expression of heat-shock proteins and antioxi-dant enzymes were analyzed by qRT-PCR after subjected to heat acclimation at 34 ℃for 48 h then 42 ℃for 12 h using heat-tolerance cultivar Han 6172 and heat-susceptible cultivar Shixin 733 .Heat tolerance of two wheat culti-vars were increased to different degrees after heat acclimation at 34℃and became marked after subjected to 42℃, Han 6172 showed more stronger heat tolerance .TaHsp70 and TaHsp16.9 genes expression could be up-regulated with different degrees in heat-tolerance cultivar after heat acclimation at 34℃too.Based on this ,the genes expres-sion were up-regulated stronger in heat-tolerance cultivar than in heat-susceptible cultivar when subjected to 42℃. Genes expression of Ta(Cu/Zn)SOD,TaMnSOD,TaApx1,TaApx4 and TaApx5 all could be up-regulated with dif-ferent degrees in heat-tolerance cultivar after heat acclimation at 34℃,and most of their expression level were high-er in heat-tolerance cultivar than that in heat-susceptible cultivar .When subjected to 42 ℃,gene expression of Ta ( Cu/Zn) SOD,TaMnSOD,TaApx1 and TaApx4 were up-regulated more stronger in heat-tolerance cultivar than that in heat-susceptible cultivar .Compared to the time point of peak ,all gene expression were not latter than that of heat killing time .Those results showed that genes of Hsp and antioxidant enzyme participated in regulating the acquisi -tion of heat tolerance during heat acclimation in wheat seedlings .The acquired heat tolerance were different in dif-ferent wheat cultivars ,the more stronger of acquired heat tolerance after heat acclimation ,the longer it can be main-tained,and the stronger heat tolerance crop showed after subjected to lethan temperature .