CAJ | 학술논문

根据肌苷合成的代谢调控机理，选择五种对肌苷合成有促进作用的代谢调控剂-氟化钠、次黄嘌呤、葡萄糖酸钙、氯化钙和柠檬酸钠，并确定了其最适添加量。进而组合添加这些代谢调控剂，对肌苷生物合成过程的多个关键代谢调控节点进行调控，增大流向肌苷合成的代谢流，使肌苷产量得到提高。研究发现，在培养基中组合添加这些代谢调控剂，所得肌苷产量比单一添加代谢调控剂时的肌苷产量有显著提升。其中当组合添加2 mg?L-1 NaF、0.15%次黄嘌呤、15 g?L-1葡萄糖酸钙及10 mg?L-1 CaCl2时，肌苷产量达到了14.01 g?L-1，相比对照组的肌苷产量提高了3.39倍，协同效应显著。继而，对肌苷合成的代谢网络各分支途径的代谢通量进行了测定，证实了组合添加代谢调控剂有利于葡萄糖代谢流由EMP途径向HMP途径发生迁移，实现对葡萄糖主体代谢流分布的调整，促使肌苷合成代谢流增加，从而有利于肌苷产量的进一步提升。
근거기감합성적대사조공궤리，선택오충대기감합성유촉진작용적대사조공제-불화납、차황표령、포도당산개、록화개화저몽산납，병학정료기최괄첨가량。진이조합첨가저사대사조공제，대기감생물합성과정적다개관건대사조공절점진행조공，증대류향기감합성적대사류，사기감산량득도제고。연구발현，재배양기중조합첨가저사대사조공제，소득기감산량비단일첨가대사조공제시적기감산량유현저제승。기중당조합첨가2 mg?L-1 NaF、0.15%차황표령、15 g?L-1포도당산개급10 mg?L-1 CaCl2시，기감산량체도료14.01 g?L-1，상비대조조적기감산량제고료3.39배，협동효응현저。계이，대기감합성적대사망락각분지도경적대사통량진행료측정，증실료조합첨가대사조공제유리우포도당대사류유EMP도경향HMP도경발생천이，실현대포도당주체대사류분포적조정，촉사기감합성대사류증가，종이유리우기감산량적진일보제승。
Following the regulating mechanism in inosine synthesis, sodium fluoride, hypoxanthine, calcium gluconate, calcium chloride and sodium citrate were used as additives for their positive effects on inosine production, and the amounts used were optimized. The key nodes in the inosine biosynthetic process were studied, and the metabolism flux was increased to promote the inosine production. The results show that the combination of these additives can increase the inosine yield which is better than that of a single additive. A combination of 0.15% hypoxanthine, 2 mg?L-1 sodium fluoride, 10 mg?L-1 calcium chloride and 15 g?L-1 calcium gluconate shows an output of 14.01 g?L-1, which is about 3.39 times higher than using any single additive. Meanwhile, measurements of the branch synthesis metabolic pathway shows that the combination of these additives does help the glucose metabolism flux moving from EMP pathway to HMP pathway, thus increases inosine biosynthesis production.