长治医学院学报
長治醫學院學報
장치의학원학보
JOURNAL OF CHANGZHI MEDICAL COLLEGE
2014年
4期
263-266,267
,共5页
陈玉芳%周林艳%万美珍%曾靖瑶
陳玉芳%週林豔%萬美珍%曾靖瑤
진옥방%주림염%만미진%증정요
hMLH1%hMSH2%COX-2%免疫组化%散发性大肠癌
hMLH1%hMSH2%COX-2%免疫組化%散髮性大腸癌
hMLH1%hMSH2%COX-2%면역조화%산발성대장암
hMLH1%hMSH2%COX-2%immunohistochemistry%sporadic colorectal carcinoma
目的：探讨错配修复基因 hMLHl、hMSH2及 COX-2蛋白在散发性大肠癌(sporadic colorectal carcinoma,SCC)中的表达及意义。方法：应用免疫组化 SP 法对60例 SCC 标本中的癌组织hMLH1、hMSH2和COX-2蛋白的表达进行检测。结果：(1)hMLHl、hMSH2在60例 SCC 组织中的阳性率为76.7%、51.7%,均较正常组有显著性差异(P <0.05)；hMLHl、hMSH2左半结肠癌阳性率为92.0%、68.0%,右半结肠癌阳性率为65.7%、40.0%,两者比较均有显著性差异(P<0.05)；淋巴结转移组中hMLH1和hMSH2蛋白阳性率分别为53.3%和26.7%,较无淋巴结转移组84.4%和60.0%均有显著性差异(P <0.05)。(2)COX-2蛋白在60例 SCC 组织中的阳性率为73.3%,较正常组阳性率(25.0%)有显著性差异(P<0.05)；COX-2蛋白淋巴结转移组中阳性率(93.3%)明显高于无淋巴结转移组66.7%,两组比较有显著性差异(P <0.05)；浸润黏膜层及肌层阳性率(55.0%)低于浆膜层表达率82.5%,两组比较有显著性差异(P<0.05)。(3)COX-2低表达的病例中 hMLH1和 hMSH2表达缺失。结论：hMLH1、hMSH2与COX-2蛋白通过不同的途径参与SCC的发生、发展。
目的：探討錯配脩複基因 hMLHl、hMSH2及 COX-2蛋白在散髮性大腸癌(sporadic colorectal carcinoma,SCC)中的錶達及意義。方法：應用免疫組化 SP 法對60例 SCC 標本中的癌組織hMLH1、hMSH2和COX-2蛋白的錶達進行檢測。結果：(1)hMLHl、hMSH2在60例 SCC 組織中的暘性率為76.7%、51.7%,均較正常組有顯著性差異(P <0.05)；hMLHl、hMSH2左半結腸癌暘性率為92.0%、68.0%,右半結腸癌暘性率為65.7%、40.0%,兩者比較均有顯著性差異(P<0.05)；淋巴結轉移組中hMLH1和hMSH2蛋白暘性率分彆為53.3%和26.7%,較無淋巴結轉移組84.4%和60.0%均有顯著性差異(P <0.05)。(2)COX-2蛋白在60例 SCC 組織中的暘性率為73.3%,較正常組暘性率(25.0%)有顯著性差異(P<0.05)；COX-2蛋白淋巴結轉移組中暘性率(93.3%)明顯高于無淋巴結轉移組66.7%,兩組比較有顯著性差異(P <0.05)；浸潤黏膜層及肌層暘性率(55.0%)低于漿膜層錶達率82.5%,兩組比較有顯著性差異(P<0.05)。(3)COX-2低錶達的病例中 hMLH1和 hMSH2錶達缺失。結論：hMLH1、hMSH2與COX-2蛋白通過不同的途徑參與SCC的髮生、髮展。
목적：탐토착배수복기인 hMLHl、hMSH2급 COX-2단백재산발성대장암(sporadic colorectal carcinoma,SCC)중적표체급의의。방법：응용면역조화 SP 법대60례 SCC 표본중적암조직hMLH1、hMSH2화COX-2단백적표체진행검측。결과：(1)hMLHl、hMSH2재60례 SCC 조직중적양성솔위76.7%、51.7%,균교정상조유현저성차이(P <0.05)；hMLHl、hMSH2좌반결장암양성솔위92.0%、68.0%,우반결장암양성솔위65.7%、40.0%,량자비교균유현저성차이(P<0.05)；림파결전이조중hMLH1화hMSH2단백양성솔분별위53.3%화26.7%,교무림파결전이조84.4%화60.0%균유현저성차이(P <0.05)。(2)COX-2단백재60례 SCC 조직중적양성솔위73.3%,교정상조양성솔(25.0%)유현저성차이(P<0.05)；COX-2단백림파결전이조중양성솔(93.3%)명현고우무림파결전이조66.7%,량조비교유현저성차이(P <0.05)；침윤점막층급기층양성솔(55.0%)저우장막층표체솔82.5%,량조비교유현저성차이(P<0.05)。(3)COX-2저표체적병례중 hMLH1화 hMSH2표체결실。결론：hMLH1、hMSH2여COX-2단백통과불동적도경삼여SCC적발생、발전。
Objective:To study the Significane and expression of hMLH1,hMSH2 and COX-2 in the development of sporadic colorectal carcinoma.Methods:Expression of hMLH1,hMSH2 and COX-2 was evaluated by immunohistochemistry in 60 SCCs.Results:(1)The positive expression rate of hMLH1、hMSH2 in SCCs was 76.7%and 51.7%,respectively.Furthermore,they are significantly lower than that in normal colorectal tissues(P<0.05).The protein expression of hMLH1 and hMLH2 in tumor tissues with lymph node metastasis was 53.3% and 26.7%,significantly higher than that(84.4%and 60.0%)in tumors without lymph node metastasis(P<0.05);the positive rate of hMLH1 and hMSH2 protein expression in the left colon was 92.0%and 68.0%,significantly higher than that in the right colon(65.7%, 40.0%).(2)The expression rate of COX-2 in SCCs was 73.3%,significantly higher than that in normal colorectal tissues(P<0.05);The positive rate of COX-2 protein expression in tumor tissues with lymph node metastasis was 93.3%,higher than that(66.7%)in tumor without lymph node metastasis.The positive rate of COX-2 protein expression in tumor tissues with mucous and muscular layer infiltration was 55.0% lower than that(82.5%)in those with serosal layer infiltration.The expression rate of COX-2 was significantly associated with lymph node metastasis(P<0.05)and with depth of infiltration(P <0.05).(3)In the tumor tissue with negative COX-2 expression, hMLH1and hMSH2 have a tendency of absent expression.Conclusion:hMLH1,hMSH2 and COX-2 are involved in genesis and development of sporadic colorectal carcinoma with defferent approach.
