CAJ | 학술논문

选用铁皮石斛兰组培苗为试验材料，以添加椰汁的MS培养基为基本培养基，研究在不同激素浓度配比条件下，对于铁皮石斛兰产生愈伤组织、诱导不定芽以及继代培养的影响，并优化铁皮石斛兰的再生条件，进而获得铁皮石斛兰离体茎尖高效扩繁再生体系。结果表明，以铁皮石斛兰的幼嫩茎尖作为试验材料，可达到高效繁殖的目的，诱导不定芽的最佳激素配比为2,4-D 0.5 mg/L和NAA 0.2 mg/L。
선용철피석곡란조배묘위시험재료，이첨가야즙적MS배양기위기본배양기，연구재불동격소농도배비조건하，대우철피석곡란산생유상조직、유도불정아이급계대배양적영향，병우화철피석곡란적재생조건，진이획득철피석곡란리체경첨고효확번재생체계。결과표명，이철피석곡란적유눈경첨작위시험재료，가체도고효번식적목적，유도불정아적최가격소배비위2,4-D 0.5 mg/L화NAA 0.2 mg/L。
Aseptic plantlets of Dendrobium officinale were selected as experimental materials to study the effect of different hormones and concentrations on callus, adventitious buds induction and subculture with MS medium added coconut milk as the basic medium. The regeneration conditions of Dendrobium ofifcinale were optimized in order to get the high efifcient regeneration system. The results showed that the regeneration ability of D. ofifcinale young shoot tip was signiifcantly higher than the other explants. The optimal hormone combination to induct callus was 2,4-D 0.5 mg/L and NAA 0.2 mg/L.