CAJ | 학술논문

目的：采用独立通风笼（individually ventilated cage， IVC）饲养实验小鼠，积累运行经验和背景数据。方法80只5周龄 SPF 级雄性 Balb/c 小鼠，在屏障内同室分别饲养在换气参数为70、50和30次/h 的 IVC 系统及敞口笼内8周，各设置5只/笼和10只/笼2种饲养密度；每周逐只称重、第8周进行大体剖检，主要脏器称重；以Excel 软件绘制体重曲线，以 SPSS 软件统计各饲养组两两间差异。结果①5只/笼饲养的动物体重曲线优于10只/笼；②与设置为30和70次/h 换气的 IVC 相比，无论是5只/笼或10只/笼饲养，换气50次/h 的 IVC 饲养动物体重曲线平滑度及趋势均更加接近5只/笼传统敞口饲养；③组间体重未因饲养设施或密度因素造成显著差异（P＞0.05）；④IVC 在30次/h 换气5只/笼饲养比敞口10只/笼饲养时的肝脏系数显著高（P ＜0.05），该参数的其它组间、及其它脏器系数值均未见组间差异显著（P ＞0.05）。结论根据实验结果，推荐该型号 IVC 换气次数设置为50次/h，饲养 Balb/c 小鼠密度5只/笼为佳。
목적：채용독립통풍롱（individually ventilated cage， IVC）사양실험소서，적루운행경험화배경수거。방법80지5주령 SPF 급웅성 Balb/c 소서，재병장내동실분별사양재환기삼수위70、50화30차/h 적 IVC 계통급창구롱내8주，각설치5지/롱화10지/롱2충사양밀도；매주축지칭중、제8주진행대체부검，주요장기칭중；이Excel 연건회제체중곡선，이 SPSS 연건통계각사양조량량간차이。결과①5지/롱사양적동물체중곡선우우10지/롱；②여설치위30화70차/h 환기적 IVC 상비，무론시5지/롱혹10지/롱사양，환기50차/h 적 IVC 사양동물체중곡선평활도급추세균경가접근5지/롱전통창구사양；③조간체중미인사양설시혹밀도인소조성현저차이（P＞0.05）；④IVC 재30차/h 환기5지/롱사양비창구10지/롱사양시적간장계수현저고（P ＜0.05），해삼수적기타조간、급기타장기계수치균미견조간차이현저（P ＞0.05）。결론근거실험결과，추천해형호 IVC 환기차수설치위50차/h，사양 Balb/c 소서밀도5지/롱위가。
Objective To accumulate operating experience and background data for housing mice in individually ventilated cages (IVC).Methods 5 weeks old Balb /c male mice(n =80) were allocated to 8 groups(n =10), which then housed in 5 or 10 per cage in 3 IVC systems(30,50 and 70 air changes /h, respectively) and one open-top cages (OTC) shelf for 8 weeks.Body weight was assessed at the initial date and every week .By the end of the experiment, necropsy was done and organs were separated and weighed .Excelland SPSS software statistics was made to draw the growth curve, and comparative analysis of body weight and organ coefficients was performed between the groups .Results 1.The growth curves of 5-mice per cage were better than that of 10-mice per cage.2.In the IVC groups, the curves trend and fluency of 50 air changes /h were more similar to that of 5-mice housed OTC group.3.The previously mentioned differences were statistically not significant (P >0.05).4.In the liver coefficients, there was a statistically significant difference between the 10-mice housed OTC group and 5-mice housed IVC group with 30 air changes /h(P <0.05), there wasn`t any other significant statistically difference with the organ coefficients between groups (P >0.05).Conclusions Based on the results of this study, the air change frequency on 50 times per hour and keeping 5 Balb/c mice per cage is recommended as the best condition for mouse housing in IVC .