CAJ | 학술논문

目的：调查不同群体树鼩弓形虫的感染情况，为建立树鼩寄生虫学监测指标提供依据。方法分别从野外来源、人工驯化一年和人工繁殖的子一代三个树鼩群体中，随机采集动物全血样本各40份，用间接血凝试验（IHA）检测弓形虫抗体，PCR 方法检测其核酸，根据两种方法的检测结果分析树鼩弓形虫的感染情况。结果IHA 和 PCR 检测结果显示，120份树鼩血样均为阴性，两种检测方法结果一致。结论本次调查三个群体来源的树鼩均未感染弓形虫，树鼩对弓形虫的易感情况还需要通过扩大样本量和感染性实验来进一步验证。
목적：조사불동군체수구궁형충적감염정황，위건립수구기생충학감측지표제공의거。방법분별종야외래원、인공순화일년화인공번식적자일대삼개수구군체중，수궤채집동물전혈양본각40빈，용간접혈응시험（IHA）검측궁형충항체，PCR 방법검측기핵산，근거량충방법적검측결과분석수구궁형충적감염정황。결과IHA 화 PCR 검측결과현시，120빈수구혈양균위음성，량충검측방법결과일치。결론본차조사삼개군체래원적수구균미감염궁형충，수구대궁형충적역감정황환수요통과확대양본량화감염성실험래진일보험증。
Objective To investigate the infection status of Toxoplasma gondii in different colonies of tree shrews and then provide the basis for parasitological monitoring .Methods Each of the forty blood samples were randomly collected from three tree shrews colonies : wild origin, domesticated and first generation, respectively.Both indirect hemagglutination test (IHA) and PCR assay were used to detect the Toxoplasma gondii.Results No positive sample of Toxoplasma gondii was detected from either IHA or PCR results .The results from IHA and PCR assays were in coincidence with each other.Conclusions According to the survey none of the tree shrews from the three groups is infected with Toxoplasma gondii.More samples or infection experiments are needed to determine whether tree shrews can be infected with Toxoplasma gondii.