CAJ | 학술논문

目的：研究猴免疫缺陷病毒（SIVmac239）多次直肠粘膜暴露对机体细胞免疫的影响。方法建立小剂量多次直肠粘膜暴露模型，测定实验猴的血浆病毒载量了解病毒复制水平，测定外周 CD4＋ T 细胞绝对数了解疾病进展情况，测定 T 细胞亚群和外周单个核细胞 IFN-γ分泌情况了解机体细胞免疫状况。结果小剂量SIVmac239病毒多次暴露能导致病毒通过直肠粘膜进入动物体内，诱导机体免疫系统出现变化，但未见 SIVmac239典型感染。病毒多次直肠暴露诱导出特异性细胞免疫，但与普通病毒感染相比，水平较低。结论本研究明确了猴免疫缺陷病毒小剂量暴露对机体细胞免疫的作用，为 HIV 疫苗研究提供了基础信息。
목적：연구후면역결함병독（SIVmac239）다차직장점막폭로대궤체세포면역적영향。방법건립소제량다차직장점막폭로모형，측정실험후적혈장병독재량료해병독복제수평，측정외주 CD4＋ T 세포절대수료해질병진전정황，측정 T 세포아군화외주단개핵세포 IFN-γ분비정황료해궤체세포면역상황。결과소제량SIVmac239병독다차폭로능도치병독통과직장점막진입동물체내，유도궤체면역계통출현변화，단미견 SIVmac239전형감염。병독다차직장폭로유도출특이성세포면역，단여보통병독감염상비，수평교저。결론본연구명학료후면역결함병독소제량폭로대궤체세포면역적작용，위 HIV 역묘연구제공료기출신식。
Objective To study the effect of repeated rectal exposure of low -dose simian immunodeficiency virus on the systemic cellular immunity in monkeys .Methods Eight 3-to 4-year old rhesus macaques ( Macaca mulatta) (male:female 1:1) were used in this study.The monkeys were inoculated with 10 TCID50 SIVmac239 virus through rectum twice a week for consecutive 6 weeks to establish a multiple rectal exposure model of SIVmac 239 virus infection.Then, plasma viral load, CD4+ T cell count, T cell subsets and IFN-γsecretion of the experiment monkeys were determined . Results Low-dose SIVmac239 virus induced some changes in the immune system through the rectal mucosa , but didn’t induce typical infection.Repeated rectal mucosal low-dose virus exposure can activate the cellular immune system . Conclusions This study defines the effect of repeated low -dose simian immunodeficiency virus exposure on the systemic cellular immunity, and provided basic information for HIV-1 vaccine research.