CAJ | 학술논문

目的：考察氧化槐果碱（ OSC ）抑制SW480细胞及诱导其产生凋亡的作用。方法根据用药剂量分组，用MTT法测定氧化槐果碱对SW480细胞增殖的影响，荧光双染观察细胞形态变化，计数凋亡细胞比率；定时定量聚合酶链式反应（ real-time PCR）检测凋亡基因caspase-3和Bcl-2的变化。结果 OSC对体外培养的SW480细胞增殖具有明显抑制作用；OSC作用后凋亡细胞比例显著增加；凋亡基因caspase-3 mRNA表达上调而Bcl-2 mRNA表达下调。结论OSC可抑制体外培养的SW480细胞生长增殖，其作用机制与阻滞细胞周期进程、诱导细胞凋亡有关。
목적：고찰양화괴과감（ OSC ）억제SW480세포급유도기산생조망적작용。방법근거용약제량분조，용MTT법측정양화괴과감대SW480세포증식적영향，형광쌍염관찰세포형태변화，계수조망세포비솔；정시정량취합매련식반응（ real-time PCR）검측조망기인caspase-3화Bcl-2적변화。결과 OSC대체외배양적SW480세포증식구유명현억제작용；OSC작용후조망세포비례현저증가；조망기인caspase-3 mRNA표체상조이Bcl-2 mRNA표체하조。결론OSC가억제체외배양적SW480세포생장증식，기작용궤제여조체세포주기진정、유도세포조망유관。
Objective To investigate oxysophocarpine inhibit prolifer-ation of human colon cancer cell line SW480 and induction of apopto-sis in which they are incurred , exploreing its mechanism at the same time.Methods Grouped according to the dose.MTT assay was used to detect the proliferation of SW480 cells.Fluorescence double stai-ning was used to observe changes in cell morphology and count the ra-tio of apoptotic cells.Flow cytometry ( FCM) were used to demonstrate the presence of apoptosis.Real -time PCR Detection were used to demonstrate changes in gene caspase-3 and Bcl-2.Results The experiment showed that OSC inhibited the growth of SW480 cells sig-nificantly.SW480 cells were induced to undergo apoptosis.Apoptotic gene caspase-3 mRNA was upregulated and Bcl-2 mRNA downreg-ulated. Conclusion Oxysophocarpine inhibits proliferation of SW480 cells in vitro, and arrest of cell cycle progression and apoptosis are involved in the mechanism.