CAJ | 학술논문

目的：研究丹酚酸B （ SalB）对脑缺血再灌注损伤大鼠炎症反应的干预作用。方法雄性Wistar大鼠随机分为3组：假手术组、模型组、实验组，制作脑中动脉闭塞模型（ MCAO），缺血1 h再灌注。分别于大鼠苏醒后、再灌注24，48 h，实验组腹腔注射SalB（12 mg· kg-1），模型组以等体积0.9％NaCl替代。在4个时间点（6，24，48，72 h）进行神经功能缺损评分，检测外周血白细胞计数、中性粒细胞绝对值，ELISA法检测血浆C反应蛋白水平（CRP），实时荧光定量 RT-PCR法检测缺血侧脑组织ICAM-1、E-selectin mRNA表达。结果 SalB可以改善模型大鼠神经功能缺损症状，降低外周血白细胞计数、中性粒细胞绝对值及血浆CRP水平，抑制缺血侧脑组织ICAM-1、E-selectin mRNA表达。结论SalB具有抗脑缺血再灌注损伤作用，其机制可能与抑制损伤后整体炎症反应水平及脑组织内黏附分子的表达有关。
목적：연구단분산B （ SalB）대뇌결혈재관주손상대서염증반응적간예작용。방법웅성Wistar대서수궤분위3조：가수술조、모형조、실험조，제작뇌중동맥폐새모형（ MCAO），결혈1 h재관주。분별우대서소성후、재관주24，48 h，실험조복강주사SalB（12 mg· kg-1），모형조이등체적0.9％NaCl체대。재4개시간점（6，24，48，72 h）진행신경공능결손평분，검측외주혈백세포계수、중성립세포절대치，ELISA법검측혈장C반응단백수평（CRP），실시형광정량 RT-PCR법검측결혈측뇌조직ICAM-1、E-selectin mRNA표체。결과 SalB가이개선모형대서신경공능결손증상，강저외주혈백세포계수、중성립세포절대치급혈장CRP수평，억제결혈측뇌조직ICAM-1、E-selectin mRNA표체。결론SalB구유항뇌결혈재관주손상작용，기궤제가능여억제손상후정체염증반응수평급뇌조직내점부분자적표체유관。
Objective To research interventional effect of salvianolic acid B ( SalB) on inflammatory reaction after cerebral ischemia reperfu-sion injury.Methods Wistar rats were randomly divided into sham operation group, model group and experiment groups.Rat Ischemia -reperfusion injury model was established by ischemia 1 h and reperfu-sion.SalB ( 12 mg · kg -1 , experiment group ) or 0.9%NaCl ( model group) were administered intraperitoneally while rats were awake from surgery and 24 h, 48 h after reperfusion.At 4 time points (6, 24, 48, 72 h after reperfusion ) , the neurological deficit scores were evaluated , while automatic blood cell analyzer detection for peripheral blood white blood cell , absolute neutrophil count .ELISA method was used to detect plasma C-reactive protein ( CRP ) levels.The real -time fluorescence quantitative RT -PCR was used to detect cerebra tissue ICAM -1, E-selectin mRNA expression.Results SalB could improve middle cer-ebral artery occlusion ( MCAO) rat neurological function and reduce the brain damage , decrease the peripheral blood white blood cell , absolute neutrophil count , plasma CRP, depress adhesion molecules including ICAM-1 , E-selectin mRNA expression in cerebral tissue.Conclusion SalB has anti -cerebral ischemia reperfusion injury effect and the mechanisms were related to overall inflammatory responses and expression of adhesion molecules after cerebral injury.