CAJ | 학술논문

本研究旨在筛选出病死动物无害化处理罐中参与动物尸体腐败的优势菌种，为研发加速动物尸体腐败的微生物制剂提供理论依据。收集病死动物无害化处理罐中动物尸体腐败液，按照10倍递增稀释方法，在参与动物尸体腐败的微生物菌群中成功筛选出一株优势菌种，采用传统分离鉴定方法和分子生物学鉴定方法对优势菌种进行鉴定。结果表明，优势腐败菌种为芽孢杆菌属的枯草芽孢杆菌，其16S rRNA基因序列与Bacillus subtilis strain 22（FJ435215.1）的同源性为99.53%。
본연구지재사선출병사동물무해화처리관중삼여동물시체부패적우세균충，위연발가속동물시체부패적미생물제제제공이론의거。수집병사동물무해화처리관중동물시체부패액，안조10배체증희석방법，재삼여동물시체부패적미생물균군중성공사선출일주우세균충，채용전통분리감정방법화분자생물학감정방법대우세균충진행감정。결과표명，우세부패균충위아포간균속적고초아포간균，기16S rRNA기인서렬여Bacillus subtilis strain 22（FJ435215.1）적동원성위99.53%。
This study aimed to screen out the dominant spoilage bacteria in the dead animal pol-lution-free disposal fermenter, which provided the theoretical basis for the development of accel-erated microbial to decompose animal corpse. Collected liquid samples from the tank, according to 10-fold increments dilution method, screened out a dominant strain in the dead animals decomposing progress, using the traditional method for isolation, identification and molecular biological iden-tification to identify the dominant strains. The results showed that the advantage bacteria was ge-nus Bacillus subtilis, which 16S rRNA gene sequence has a 99.53% homology with Bacillus subtilis strain 22(FJ435215.1).