现代畜牧兽医
現代畜牧獸醫
현대축목수의
LIAONING JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY MEDICINE
2014年
8期
1-4
,共4页
陈生雷%李凤艳%高洪霞%李沂%王岩%舒秀伟
陳生雷%李鳳豔%高洪霞%李沂%王巖%舒秀偉
진생뢰%리봉염%고홍하%리기%왕암%서수위
活疫苗%耐热保护剂%热稳定性试验
活疫苗%耐熱保護劑%熱穩定性試驗
활역묘%내열보호제%열은정성시험
Live vaccine%Thermostable agent%Thermostablility test
应用冻干制品加速热稳定试验,测试7组冻干保护剂配方对鸡新城疫、传染性支气管炎二联活疫苗(La Sota株+H120株)的耐热保护效果。试验筛选出用于鸡新城疫、传染性支气管炎二联活疫苗的耐热保护剂,使用该保护剂的疫苗表现为37℃保存10 d,病毒含量下降0.4个滴度,常规疫苗下降2~2.4个滴度;25℃保存30 d,病毒含量下降0.4~0.6个滴度,常规疫苗下降1.8~2.6个滴度。证明此保护剂具有良好的耐热效果。
應用凍榦製品加速熱穩定試驗,測試7組凍榦保護劑配方對鷄新城疫、傳染性支氣管炎二聯活疫苗(La Sota株+H120株)的耐熱保護效果。試驗篩選齣用于鷄新城疫、傳染性支氣管炎二聯活疫苗的耐熱保護劑,使用該保護劑的疫苗錶現為37℃保存10 d,病毒含量下降0.4箇滴度,常規疫苗下降2~2.4箇滴度;25℃保存30 d,病毒含量下降0.4~0.6箇滴度,常規疫苗下降1.8~2.6箇滴度。證明此保護劑具有良好的耐熱效果。
응용동간제품가속열은정시험,측시7조동간보호제배방대계신성역、전염성지기관염이련활역묘(La Sota주+H120주)적내열보호효과。시험사선출용우계신성역、전염성지기관염이련활역묘적내열보호제,사용해보호제적역묘표현위37℃보존10 d,병독함량하강0.4개적도,상규역묘하강2~2.4개적도;25℃보존30 d,병독함량하강0.4~0.6개적도,상규역묘하강1.8~2.6개적도。증명차보호제구유량호적내열효과。
In this paper, we tested protective effects of 7 groups of the lyoprotectant recipe for Newcastle Disease and Infectious Bronchitis Vaccine, Live Vaccine (La Sota strain + H120 strain) were used for cross reference by an accelerated stability test. The best lyoprotectant rec-ipe of Newcastle-Bronchitis Vaccine had been screened in this experiment, and the results indicat-ed that when exposure this lyoprotectant vaccine at 37 ℃ for 10 days, or exposure at 25 ℃ for 30 days, the vaccine virus titers declined by 0.4 unit or 0.4~0.6 unit respectively, while other lyo-protectant vaccines virus titers decreased by 2~2.4 unit or 1.8~2.6 unit respectively. In conclu-sion, the new lyoprotectant showed effective protection for Newcastle-Bronchitis Vaccine.