CAJ | 학술논문

建立了以L-Cys-CdS纳米荧光探针测定芦丁含量的荧光分析方法。以L-半胱氨酸为稳定剂,在水溶液中合成了CdS纳米荧光探针,其粒径约为20nm。芦丁对CdS纳米荧光探针有荧光猝灭作用,且其紫外光谱图与变温实验表明其为静态猝灭。考察了酸度、反应时间、干扰物质对该方法的影响。在体系pH为10．5时,该方法的线性范围为2．004~48．096μg·mL-1,r=0．9992,检出限为0．672μg·mL-1,精密度为1．2%。该方法已成功用于复方芦丁片中芦丁的含量测定,与中国药典中的标准方法比较,结果满意。该方法简便,快捷,可用于芦丁含量的测定。
건립료이L-Cys-CdS납미형광탐침측정호정함량적형광분석방법。이L-반광안산위은정제,재수용액중합성료CdS납미형광탐침,기립경약위20nm。호정대CdS납미형광탐침유형광졸멸작용,차기자외광보도여변온실험표명기위정태졸멸。고찰료산도、반응시간、간우물질대해방법적영향。재체계pH위10．5시,해방법적선성범위위2．004~48．096μg·mL-1,r=0．9992,검출한위0．672μg·mL-1,정밀도위1．2%。해방법이성공용우복방호정편중호정적함량측정,여중국약전중적표준방법비교,결과만의。해방법간편,쾌첩,가용우호정함량적측정。
Determination of rutin content by fluorometry was carried out. In this experiment,the Cds nano-fluorescent probe was pre-pared using L-Cys as stabilizer in aqueous solution. Its particle size is about 20 nm. Based on the decrease of fluorescence intensity after L-Cys-CdS reacted with rutin,a new method for the determination of rutin was established. The uv spectra and variable temper-ature experiment showed that the quenching process was static. Some experiment influencing factors were studied such as acidity,re-action time,and interfering substance. The results showed that the fluorescence intensity of system with pH 10. 5 had a good linear relationship with the concentration of rutin in the range of 2. 004~48. 096μg·mL-1(r=0. 9992). The detection limit was 0. 672μg ·mL-1 and the precision was 1. 2%. The method has been applied to the determination of rutin in compound rutin tablets,and com-pared with the standard method( HPLC) ,the results were satisfactory. The method presented here is simple,rapid and can be used for the determination of rutin.