CAJ | 학술논문

对采自云南丽江17个不同产区秦艽样品建立其HPLC指纹图谱，测定了药材中9大质量指标并进行灰色关联度分析，建立一种新型秦艽质量综合评价方法。采用甲醇-0．1%磷酸为流动相体系，梯度洗脱，建立17批秦艽药材的高效液相色谱指纹图谱；采用HPLC法测定马钱苷酸，龙胆苦苷和獐牙菜苦苷含量；按药典方法测秦艽中醇溶性浸出物、总灰分、酸不溶性灰分含量；分光光度法测定多糖含量。建立的丽江秦艽药材的HPLC指纹图谱的相似度极高，通过对多项指标的灰色关联度分析遴选出X2和X12两个优级药材资源。指纹图谱结合多指标成分的灰色关联度分析，能全面评价秦艽药材质量，亦可从中筛选优质秦艽资源和为秦艽道地适宜产区的规划确定提供依据。
대채자운남려강17개불동산구진구양품건립기HPLC지문도보，측정료약재중9대질량지표병진행회색관련도분석，건립일충신형진구질량종합평개방법。채용갑순-0．1%린산위류동상체계，제도세탈，건립17비진구약재적고효액상색보지문도보；채용HPLC법측정마전감산，룡담고감화장아채고감함량；안약전방법측진구중순용성침출물、총회분、산불용성회분함량；분광광도법측정다당함량。건립적려강진구약재적HPLC지문도보적상사도겁고，통과대다항지표적회색관련도분석린선출X2화X12량개우급약재자원。지문도보결합다지표성분적회색관련도분석，능전면평개진구약재질량，역가종중사선우질진구자원화위진구도지괄의산구적규화학정제공의거。
This paper aims to establish a new comprehensive method to evaluation the quality of gentiana macrophylla which collect-ed from 17 different regions in LIjiang. We established HPLC fingerprint and tested 9 quality indexs of the samples. HPLC finger-print was established with mobile phase consisting of methanol and 0. 1%phosphoric acid;The loganic acid,swertiamarin and genti-opicroside were detected by HPLC method;The ethanol-soluble extractives,total ash and acid insoluble ash were determined accord-ing to the pharmacopoeia method;Spectrophotometric method was used to determine polysaccharide content. HPLC fingerprint of the samples had high similarity. The grey relational analysis showed that X2 and X12 were two most excellent medicinal material. Finger-print analysis and grey relational analysis combined can comprehensively evaluate the quality of gentianae macrophyllae and can se-lect high quality of gentianae macrophyllae resources. This paper provided the basis for determination gentiana macrophylla authentic suitable planting region.