CAJ | 학술논문

对哈尔滨地区规模化猪场沙门氏菌进行分离鉴定，分析其对β-内酰胺类药物的药敏试验结果及耐药情况。采用病原菌的形态学观察、生化鉴定和PCR鉴定，从600份哈尔滨地区规模化猪场猪直肠粪便中分离出沙门氏菌，运用Kirby-Bauer法检测分离菌对10种β-内酰胺类药物的敏感性。结果表明，在哈尔滨地区十市县的猪群中，沙门氏菌的总检出率为5.83%（35/600）；不同市县沙门氏菌的总分离率介于3.3%~18.3%；沙门氏菌的分离率与猪日龄有一定关系；对临床常用抗菌药物均表现出较高的耐药性，对氨苄西林100%耐药，对头孢他啶80%耐药，对头孢西丁、哌拉西林、头孢噻肟、头孢吡肟、氨曲南存在不同程度耐药，对碳青霉烯类敏感。结果表明，哈尔滨地区猪场存在不同程度的沙门氏菌感染，耐药现象严重。
대합이빈지구규모화저장사문씨균진행분리감정，분석기대β-내선알류약물적약민시험결과급내약정황。채용병원균적형태학관찰、생화감정화PCR감정，종600빈합이빈지구규모화저장저직장분편중분리출사문씨균，운용Kirby-Bauer법검측분리균대10충β-내선알류약물적민감성。결과표명，재합이빈지구십시현적저군중，사문씨균적총검출솔위5.83%（35/600）；불동시현사문씨균적총분리솔개우3.3%~18.3%；사문씨균적분리솔여저일령유일정관계；대림상상용항균약물균표현출교고적내약성，대안변서림100%내약，대두포타정80%내약，대두포서정、고랍서림、두포새우、두포필우、안곡남존재불동정도내약，대탄청매희류민감。결과표명，합이빈지구저장존재불동정도적사문씨균감염，내약현상엄중。
Serological identification of Salmonella in the large-scale pig farms of Haerbin, and analyzed the different Salmonella antimicrobial susceptibility testresults and drug resistance.Methods Up to 35 Salmonella strains were isolated from 600 feces in the rectal of pigs by morphological observation and biochemical system. The sensitivity tests of 35 strains to 10 Beta lactam antibacterials were performed by Krby-Bauer method.These microorganisms were further confirmed by correspond-ing specific bio-chemistry assay and polymerase chain reaction (PCR). The reasults showed that the overall detection rate of Salmonella was 5.83%and the total rate of separation was from 3.3%to 18.3%in the different citys of Harbin. The resistant rate of 35 strains to ampicillin, ceftazidime, cefoxitin, piperacillin, cefotaxime, cefepime and aztreonam was 100%, 80%, 17%, 71%, 66%, 6 3% and 29%respectively. However, they were still sensitive to ertapenem,meropenem and imipenem. The results showed that Salmonella infections exist in pig farms in Harbin and the antimicrobial resistance of Salmonella is very serious.