CAJ | 학술논문

目的：采用傅里叶变换红外光谱技术结合系统聚类分析对云南产石斛进行研究。方法用傅里叶变换红外光谱法测试了11个品种83株石斛样品的红外光谱，并结合二阶导数光谱对比分析光谱信息差异，用相关分析和系统聚类分析进行品种差异和鉴别分析。结果光谱信息显示，石斛中的主要成分是纤维素和木质素等多糖物质；铁皮石斛中纤维素和木质素的含量相对较小，其他多糖物质成分及含量相对较多。测试石斛品种的相关性较高，红外光谱的相关系数均在0.980以上，二阶导数光谱的相关系数在0.751～0.980之间。用二阶导数光谱进行系统聚类，分类正确率达87.9%；系统聚类显示重唇石斛、罗河石斛与钩状石斛，长苏石斛与金钗石斛，滇桂石斛与流苏石斛的物质组分更相近。结论红外光谱结合系统聚类分析可以区分不同品种石斛的物质组分差异，鉴别不同品种的石斛，具有方便快捷的特点。
목적：채용부리협변환홍외광보기술결합계통취류분석대운남산석곡진행연구。방법용부리협변환홍외광보법측시료11개품충83주석곡양품적홍외광보，병결합이계도수광보대비분석광보신식차이，용상관분석화계통취류분석진행품충차이화감별분석。결과광보신식현시，석곡중적주요성분시섬유소화목질소등다당물질；철피석곡중섬유소화목질소적함량상대교소，기타다당물질성분급함량상대교다。측시석곡품충적상관성교고，홍외광보적상관계수균재0.980이상，이계도수광보적상관계수재0.751～0.980지간。용이계도수광보진행계통취류，분류정학솔체87.9%；계통취류현시중진석곡、라하석곡여구상석곡，장소석곡여금차석곡，전계석곡여류소석곡적물질조분경상근。결론홍외광보결합계통취류분석가이구분불동품충석곡적물질조분차이，감별불동품충적석곡，구유방편쾌첩적특점。
Objective To study Dendrobium from Yunnan Province by Fourier transform infrared spectroscopy (FTIR) combined with hierarchical cluster analysis.Methods Stems of 83 trees from 11 Dendrobium varieties were tested through using FTIR, so as to analyze the differences among different varieties and identify Dendrobium variety by second-derivative spectral, the correlation analysis and hierarchical cluster analysis.Results The spectra showed that the main ingredients of stems were cellulose, lignin and polysaccharides. The fraction of lignin and cellulose ofD.officinate was lower, while that for polysaccharides was higher. There was a high correlation among spectra, with the correlation coefficients of more than 0.980, and that for second-derivative spectral was 0.751 to 0.980. All the samples were clustered by second-derivative spectral, with the correct rate of 87.9%. Hierarchical cluster analysis showed that the components ofD.hercoglossum, D.lohohense andD.adumcum,D.brymerianum andD.nobile,D.guangxiense andD.fimbriatum are more similar than that of others.Conclusion FTIR combined with hierarchical cluster analysis can distinguish and identify different varieties of Dendrobium, and is an effective and simple way to identify different varieties.